Difference between revisions of "PMID:363701"

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{|  id="W4fb5641a51e80"  class=" tableEdit PMID_info_table" 
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!align=left  |Citation
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'''Dabbs, ER'''  (1978) Kasugamycin-dependent mutants of Escherichia coli.''J. Bacteriol.'' '''136''':994-1001
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!align=left  |Abstract
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Kasugamycin-dependent mutants have been isolated from Escherichia coli B. They were obtained through mutagenesis with ethyl methane sulfonate or nitrosoguanidine in conjunction with an antibiotic underlay technique. In the case of nitrosoguanidine, dependent mutants were obtained at a frequency of about 3% of survivors growing up in the selection. In the case of ethyl methane sulfonate, the corresponding value was 1%. Nineteen mutants showing a kasugamycin-dependent phenotype were studied. In terms of response to various temperatures and antibiotic concentrations, they were very heterogeneous, although most fell into two general classes. Genetic analysis indicated that in at least some cases, the kasugamycin-dependent phenotype was the product of two mutations. Two-dimensional gel electropherograms revealed alterations in the ribosomal proteins of seven mutants. One mutant had an alteration in protein S13, and one had an alteration in protein L14. Three showed changes in protein S9. Each of two mutants had changes in two proteins, S18 and L11. Three of these mutants additionally had protein S18 occurring in a partly altered, partly unaltered form.
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!align=left  |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=363701 PubMed]
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!align=left  |Keywords
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Aminoglycosides; Anti-Bacterial Agents; Bacterial Proteins; Chromosome Mapping; Chromosomes, Bacterial; Dose-Response Relationship, Drug; Escherichia coli; Genes; Mutation; Ribosomal Proteins; Temperature
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==Main Points of the Paper ==
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{{LitSignificance}}
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== Materials and Methods Used ==
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{{LitMaterials}}
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==Phenotype Annotations==
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{{AnnotationTableHelp}}
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="B4fb5641a5574e"  class=" tableEdit Phenotype_Table_2" 
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|- align='left' bgcolor='#CCCCFF'
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!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
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*Strain: B
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*Substrain: L44
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*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=37762 37762]
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*Genotype of Reference Strain: ''rpsR<sup>+</sup>''
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*Genotype of Experimental Strain : ''Ksg <sup>r</sup> rpsR MV9''
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*Reference Condition:
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0000276
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|
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increased resistance to antimicrobial compound
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|
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0000181
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|
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in vitro assay data
 +
|
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Kasugamycin resistance developed when the strain was grown 42C.
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|
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|-
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|
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a mutation or genetic difference within a strain
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|
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*Taxon: Escherichia coli
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*Strain: B
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*Substrain: L44
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*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=37762 37762]
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|
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*Genotype of Reference Strain: ''rpsR<sup>+</sup>''
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*Genotype of Experimental Strain : ''Ksg <sup>d</sup> rpsR MV9''
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|
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*Reference Condition:
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|
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0005101
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|
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aminoglycoside dependent growth
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|
 +
0000181
 +
|
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in vitro assay data
 +
|
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Kasugamycin dependence developed when the strain was grown 30C. See Figure 2b for gel electropherogram. Strains MV 2, 29, 34 and 35 had were also Ksg<sup>d</sup>, see table 1.
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|
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|-
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|
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a mutation or genetic difference within a strain
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|
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*Taxon: Escherichia coli
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*Strain: B
 +
*Substrain: L44
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=37762 37762]
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|
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*Genotype of Reference Strain: ''rpsM<sup>+</sup>
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*Genotype of Experimental Strain : ''Ksg <sup>d</sup> rpsM MV34''
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|
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*Reference Condition:
 +
|
 +
0005101
 +
|
 +
aminoglycoside dependent growth
 +
|
 +
0000181
 +
|
 +
in vitro assay data
 +
|
 +
Kasugamycin dependence developed when the strain was grown 30C. See Figure 2E for gel electropherogram of 70S ribosome protein.
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: B
 +
*Substrain: L44
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=37762 37762]
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|
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*Genotype of Reference Strain: ''rpsI<sup>+</sup>''
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*Genotype of Experimental Strain : ''Ksg<sup>d</sup> rpsI MV9''
 +
|
 +
*Reference Condition:
 +
|
 +
0005101
 +
|
 +
aminoglycoside dependent growth
 +
|
 +
0000181
 +
|
 +
in vitro assay data
 +
|
 +
Kasugamycin dependence was dependent on the temperature it was grown at; 30 C. See table 1 and figure 2b.  Strain MV21 had similar results see figure 2c.
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: B
 +
*Substrain: L44
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=37762 37762]
 +
|
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*Genotype of Reference Strain: ''Ksg<sup>s</sup> rpsR<sup>+</sup> L44''
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*Genotype of Experimental Strain : ''Ksg<sup>d</sup> rpsR<sup>-</sup> MV3''
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|
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*Reference Condition:
 +
|
 +
0005101
 +
|
 +
aminoglycoside dependent growth
 +
|
 +
0000033
 +
|
 +
traceable author statement
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|
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MV3 was found to be Kasugamycin dependent as well as have growth which was independent of temperature.  See page 995 for full list of similar mutant strains.
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: B
 +
*Substrain: L44
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=37762 37762]
 +
|
 +
*Genotype of Reference Strain: ''prmA<sup>+</sup>''
 +
*Genotype of Experimental Strain : ''Ksg<sup>d</sup> prmA MV9''
 +
|
 +
*Reference Condition:
 +
|
 +
0005101
 +
|
 +
aminoglycoside dependent growth
 +
|
 +
0000181
 +
|
 +
in vitro assay data
 +
|
 +
Kasugamycin dependence developed. See Figure 2 for partial to dimensional gel electropherogram of the 70S ribosomal protein. 
 +
|
 +
 +
|-
 +
|
 +
a mutation or genetic difference within a strain
 +
|
 +
*Taxon: Escherichia coli
 +
*Strain: B
 +
*Substrain: L44
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=37762 37762]
 +
|
 +
*Genotype of Reference Strain: ''Ksg<sup>s</sup> rpsR<sup>+</sup> L44''
 +
*Genotype of Experimental Strain : ''Ksg<sup>d</sup> rpsR<sup>-</sup> MV4''
 +
|
 +
*Reference Condition:
 +
|
 +
0005101
 +
|
 +
aminoglycoside dependent growth
 +
|
 +
0000033
 +
|
 +
traceable author statement
 +
|
 +
MV4 was found to be Kasugamycin dependent but with growth that was temperature dependent.  See page 995 for full list of similar mutant strains.
 +
|
 +
 +
 +
|- class="tableEdit_footer"
 +
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.3178.B4fb5641a5574e&page=3178&pagename={{FULLPAGENAMEE}}&type=0&template=Phenotype_Table_2 edit table]</span> || || || || || || || || ||
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|}
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<!--box uid=d41d8cd98f00b204e9800998ecf8427e.3178.B4fb5641a5574e--></protect>
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==Notes==
 +
*There are mutations mentioned that were not clearly linked to any genes.
 +
 +
==References==
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{{RefHelp}}
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<references/>
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[[Category:Publication]]  [[Category:Papers referenced in the LaRossa chapter]]

Latest revision as of 17:28, 12 February 2014

Citation

Dabbs, ER (1978) Kasugamycin-dependent mutants of Escherichia coli.J. Bacteriol. 136:994-1001

Abstract

Kasugamycin-dependent mutants have been isolated from Escherichia coli B. They were obtained through mutagenesis with ethyl methane sulfonate or nitrosoguanidine in conjunction with an antibiotic underlay technique. In the case of nitrosoguanidine, dependent mutants were obtained at a frequency of about 3% of survivors growing up in the selection. In the case of ethyl methane sulfonate, the corresponding value was 1%. Nineteen mutants showing a kasugamycin-dependent phenotype were studied. In terms of response to various temperatures and antibiotic concentrations, they were very heterogeneous, although most fell into two general classes. Genetic analysis indicated that in at least some cases, the kasugamycin-dependent phenotype was the product of two mutations. Two-dimensional gel electropherograms revealed alterations in the ribosomal proteins of seven mutants. One mutant had an alteration in protein S13, and one had an alteration in protein L14. Three showed changes in protein S9. Each of two mutants had changes in two proteins, S18 and L11. Three of these mutants additionally had protein S18 occurring in a partly altered, partly unaltered form.

Links

PubMed

Keywords

Aminoglycosides; Anti-Bacterial Agents; Bacterial Proteins; Chromosome Mapping; Chromosomes, Bacterial; Dose-Response Relationship, Drug; Escherichia coli; Genes; Mutation; Ribosomal Proteins; Temperature

edit table

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: B
  • Substrain: L44
  • NCBI Taxon ID: 37762
  • Genotype of Reference Strain: rpsR+
  • Genotype of Experimental Strain : Ksg r rpsR MV9
  • Reference Condition:

0000276

increased resistance to antimicrobial compound

0000181

in vitro assay data

Kasugamycin resistance developed when the strain was grown 42C.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: B
  • Substrain: L44
  • NCBI Taxon ID: 37762
  • Genotype of Reference Strain: rpsR+
  • Genotype of Experimental Strain : Ksg d rpsR MV9
  • Reference Condition:

0005101

aminoglycoside dependent growth

0000181

in vitro assay data

Kasugamycin dependence developed when the strain was grown 30C. See Figure 2b for gel electropherogram. Strains MV 2, 29, 34 and 35 had were also Ksgd, see table 1.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: B
  • Substrain: L44
  • NCBI Taxon ID: 37762
  • Genotype of Reference Strain: rpsM+
  • Genotype of Experimental Strain : Ksg d rpsM MV34
  • Reference Condition:

0005101

aminoglycoside dependent growth

0000181

in vitro assay data

Kasugamycin dependence developed when the strain was grown 30C. See Figure 2E for gel electropherogram of 70S ribosome protein.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: B
  • Substrain: L44
  • NCBI Taxon ID: 37762
  • Genotype of Reference Strain: rpsI+
  • Genotype of Experimental Strain : Ksgd rpsI MV9
  • Reference Condition:

0005101

aminoglycoside dependent growth

0000181

in vitro assay data

Kasugamycin dependence was dependent on the temperature it was grown at; 30 C. See table 1 and figure 2b. Strain MV21 had similar results see figure 2c.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: B
  • Substrain: L44
  • NCBI Taxon ID: 37762
  • Genotype of Reference Strain: Ksgs rpsR+ L44
  • Genotype of Experimental Strain : Ksgd rpsR- MV3
  • Reference Condition:

0005101

aminoglycoside dependent growth

0000033

traceable author statement

MV3 was found to be Kasugamycin dependent as well as have growth which was independent of temperature. See page 995 for full list of similar mutant strains.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: B
  • Substrain: L44
  • NCBI Taxon ID: 37762
  • Genotype of Reference Strain: prmA+
  • Genotype of Experimental Strain : Ksgd prmA MV9
  • Reference Condition:

0005101

aminoglycoside dependent growth

0000181

in vitro assay data

Kasugamycin dependence developed. See Figure 2 for partial to dimensional gel electropherogram of the 70S ribosomal protein.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: B
  • Substrain: L44
  • NCBI Taxon ID: 37762
  • Genotype of Reference Strain: Ksgs rpsR+ L44
  • Genotype of Experimental Strain : Ksgd rpsR- MV4
  • Reference Condition:

0005101

aminoglycoside dependent growth

0000033

traceable author statement

MV4 was found to be Kasugamycin dependent but with growth that was temperature dependent. See page 995 for full list of similar mutant strains.


edit table

</protect>

Notes

  • There are mutations mentioned that were not clearly linked to any genes.

References

See Help:References for how to manage references in omp dev.

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