Difference between revisions of "PMID:20519959"

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'''Spirek, M, Benko, Z, Carnecka, M, Rumpf, C, Cipak, L, Batova, M, Marova, I, Nam, M, Kim, DU, Park, HO, Hayles, J, Hoe, KL, Nurse, P and Gregan, J'''  (2010) S. pombe genome deletion project: an update. ''Cell Cycle'' '''9''':2399-402
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!align=left align='left' bgcolor='#CCCCFF' |Abstract
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The fission yeast Schizosaccharomyces pombe is a model organism used widely to study various aspects of eukaryotic biology. A collection of heterozygous diploid strains containing individual deletions in nearly all S. pombe genes has been created using a PCR based strategy. However, deletion of some genes has not been possible using this methodology. Here we use an efficient knockout strategy based on plasmids that contain large regions homologous to the target gene to delete an additional 29 genes. The collection of deletion mutants now covers 99% of the fission yeast open reading frames.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=20519959 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3132452 PMC3132452]
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Online version:[http://dx.doi.org/10.4161/cc.9.12.11914 10.4161/cc.9.12.11914]
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!align=left align='left' bgcolor='#CCCCFF' |Keywords
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DNA Restriction Enzymes; Gene Deletion; Gene Knockout Techniques/methods; Genetic Vectors; Genome, Fungal; Open Reading Frames; Plasmids; Polymerase Chain Reaction; Schizosaccharomyces/genetics
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==Main Points of the Paper ==
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== Materials and Methods Used ==
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==Phenotype Annotations==
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==Notes==
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==References==
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[[Category:Publication]]

Latest revision as of 21:26, 15 April 2017

Citation

Spirek, M, Benko, Z, Carnecka, M, Rumpf, C, Cipak, L, Batova, M, Marova, I, Nam, M, Kim, DU, Park, HO, Hayles, J, Hoe, KL, Nurse, P and Gregan, J (2010) S. pombe genome deletion project: an update. Cell Cycle 9:2399-402

Abstract

The fission yeast Schizosaccharomyces pombe is a model organism used widely to study various aspects of eukaryotic biology. A collection of heterozygous diploid strains containing individual deletions in nearly all S. pombe genes has been created using a PCR based strategy. However, deletion of some genes has not been possible using this methodology. Here we use an efficient knockout strategy based on plasmids that contain large regions homologous to the target gene to delete an additional 29 genes. The collection of deletion mutants now covers 99% of the fission yeast open reading frames.

Links

PubMed PMC3132452 Online version:10.4161/cc.9.12.11914

Keywords

DNA Restriction Enzymes; Gene Deletion; Gene Knockout Techniques/methods; Genetic Vectors; Genome, Fungal; Open Reading Frames; Plasmids; Polymerase Chain Reaction; Schizosaccharomyces/genetics

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Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status
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Notes

References

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