Difference between revisions of "PMID:4927942"

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!align=left  |Keywords
 
!align=left  |Keywords
 
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Acridines; Bromides; Cell Division; Chromosomes, Bacterial; Coliphages; DNA; DNA Replication; Escherichia coli; Extrachromosomal Inheritance; Genetics, Microbial; Mutation; Quaternary Ammonium Compounds; Quinolines; Temperature
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Integrative Suppression; Hfr; Phage; Acridine Orange; Ethidium Bromide; DNA Replication; Reversion; Temperature-sensitivity
  
 
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==Main Points of the Paper ==
 
==Main Points of the Paper ==
*The chromosome may be incorporated into the F replicon in Hfr strains
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*New type of suppression of a temperature-sensitive mutation of DNA replication- the chromosome may be incorporated into the F replicon in Hfr strains
  
 
== Materials and Methods Used ==
 
== Materials and Methods Used ==
{{LitMaterials}}
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*Acridine orange sensitivity assay
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*EtBr sensitivity assay
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*Cross-streak test for conjugation
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*Transduction
  
 
==Phenotype Annotations==
 
==Phenotype Annotations==
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!|Species!!Taxon ID!!Strain!!Gene (if known)!!OMP!!Phenotype!!Details!!Evidence!!Notes
 
!|Species!!Taxon ID!!Strain!!Gene (if known)!!OMP!!Phenotype!!Details!!Evidence!!Notes
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''Escherichia coli''
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NCBI:83333
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CRT46
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*slow stop DNA replication defect
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*abolished initiation of DNA replication at high temperature
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Biochemical Assay
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''Escherichia coli''
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NCBI:83333
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CRT226
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*fast stop DNA replication defect
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*abolished DNA replication at high temperature
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Biochemical Assay
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''Escherichia coli''
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NCBI:83333
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PAT42
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*fast stop DNA replication defect
 +
*abolished DNA replication at high temperature
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Biochemical Assay
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{{RefHelp}}
 
{{RefHelp}}
 
<references/>
 
<references/>
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[[Category:Publication]]
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[[Category:To Be Converted]]

Latest revision as of 12:09, 22 June 2011

Citation

Nishimura, Y, Caro, L, Berg, CM and Hirota, Y (1971) Chromosome replication in Escherichia coli. IV. Control of chromosome replication and cell division by an integrated episome.J. Mol. Biol. 55:441-56

Abstract

No abstract in PubMed

Links

PubMed

Keywords

Integrative Suppression; Hfr; Phage; Acridine Orange; Ethidium Bromide; DNA Replication; Reversion; Temperature-sensitivity

Main Points of the Paper

  • New type of suppression of a temperature-sensitive mutation of DNA replication- the chromosome may be incorporated into the F replicon in Hfr strains

Materials and Methods Used

  • Acridine orange sensitivity assay
  • EtBr sensitivity assay
  • Cross-streak test for conjugation
  • Transduction

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Species Taxon ID Strain Gene (if known) OMP Phenotype Details Evidence Notes

Escherichia coli

NCBI:83333

CRT46

  • slow stop DNA replication defect
  • abolished initiation of DNA replication at high temperature

Biochemical Assay

Escherichia coli

NCBI:83333

CRT226

  • fast stop DNA replication defect
  • abolished DNA replication at high temperature

Biochemical Assay

Escherichia coli

NCBI:83333

PAT42

  • fast stop DNA replication defect
  • abolished DNA replication at high temperature

Biochemical Assay


</protect>

Notes

References

See Help:References for how to manage references in omp dev.