PMID:9973363

Citation	Dermić, D and Trgovcević, Z (1999) Specific effects of a recB mutation on the HfrH strain of Escherichia coli.J. Bacteriol. 181:1334-7
Abstract	The recB268::Tn10 mutation was introduced into the HfrH strain of Escherichia coli. Compared with recB F- and recB F+ cells, the viability of this mutant strain was much lower. Compared with wild-type HfrH, the recB derivative donated much shorter fragments of its chromosome to the recipient. It is suggested that the recB gene product (i.e., RecBCD enzyme) participates in Hfr transfer.
Links	PubMed
Keywords	Conjugation, Genetic; Escherichia coli; Escherichia coli Proteins; Exodeoxyribonuclease V; Exodeoxyribonucleases; F Factor; Mutation
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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: '3000HfrH' NCBI Taxon ID: 83333	Genotype of Reference Strain: rec+HfrH Genotype of Experimental Strain : recBHfrH	Reference Condition:					After the insertion of the Tn10 mutation the IRB101 cells grew extremely slowly. The generation time being four to five times longer than the parental strain's.
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