Difference between revisions of "PMID:7009552"
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| + | {| id="H4f70e7510f509" class=" tableEdit PMID_info_table" | ||
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| + | !align=left |Citation | ||
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| + | '''Platz, A and Sjöberg, BM''' (1980) Construction and characterization of hybrid plasmids containing the Escherichia coli nrd region.''J. Bacteriol.'' '''143''':561-8 | ||
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| + | !align=left |Abstract | ||
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| + | Recombinant plasmids containing all or part of the genetic region of Escherichia coli coding for the two subunits of ribonucleoside diphosphate reductase (proteins B1 and B2) were constructed with the aid of the multicopy plasmid pBR322. Two of these plasmids (pPS1 and pPS2) appeared to carry both a regulator and the complete structural information for the enzyme and, after transformation of E. coli, directed a 10- to 20-fold overproduction of both proteins B1 and B2. The other plasmids (pPS101 and pPS201) carried structural information for only protein B2. Cells carrying pPS1 and pPS2 showed a 5- to 500-fold increased resistance against the drug hydroxyurea. This establishes that in E. coli the inhibition of deoxyribonucleic acid synthesis by hydroxyurea is fully explained by its action on ribonucleotide reductase. | ||
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| + | !align=left |Links | ||
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| + | [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7009552 PubMed] | ||
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| + | !align=left |Keywords | ||
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| + | DNA, Recombinant; Enzyme Induction; Escherichia coli; Genes; Hydroxyurea; Macromolecular Substances; Plasmids; Ribonucleoside Diphosphate Reductase; Ribonucleotide Reductases | ||
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| + | <!--box uid=d41d8cd98f00b204e9800998ecf8427e.2894.H4f70e7510f509--> | ||
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| + | ==Main Points of the Paper == | ||
| + | {{LitSignificance}} | ||
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| + | == Materials and Methods Used == | ||
| + | {{LitMaterials}} | ||
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| + | ==Phenotype Annotations== | ||
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| + | !|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status | ||
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| + | ==Notes== | ||
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| + | ==References== | ||
| + | {{RefHelp}} | ||
| + | <references/> | ||
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| + | [[Category:Publication]] | ||
Revision as of 17:01, 26 March 2012
| Citation |
Platz, A and Sjöberg, BM (1980) Construction and characterization of hybrid plasmids containing the Escherichia coli nrd region.J. Bacteriol. 143:561-8 |
|---|---|
| Abstract |
Recombinant plasmids containing all or part of the genetic region of Escherichia coli coding for the two subunits of ribonucleoside diphosphate reductase (proteins B1 and B2) were constructed with the aid of the multicopy plasmid pBR322. Two of these plasmids (pPS1 and pPS2) appeared to carry both a regulator and the complete structural information for the enzyme and, after transformation of E. coli, directed a 10- to 20-fold overproduction of both proteins B1 and B2. The other plasmids (pPS101 and pPS201) carried structural information for only protein B2. Cells carrying pPS1 and pPS2 showed a 5- to 500-fold increased resistance against the drug hydroxyurea. This establishes that in E. coli the inhibition of deoxyribonucleic acid synthesis by hydroxyurea is fully explained by its action on ribonucleotide reductase. |
| Links | |
| Keywords |
DNA, Recombinant; Enzyme Induction; Escherichia coli; Genes; Hydroxyurea; Macromolecular Substances; Plasmids; Ribonucleoside Diphosphate Reductase; Ribonucleotide Reductases |
| edit table |
Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
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<protect>
| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
| edit table |
</protect>
Notes
References
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