PMID:7028117

Citation	Slater, AC, Jones-Mortimer, MC and Kornberg, HL (1981) L-Sorbose phosphorylation in Escherichia coli K-12.Biochim. Biophys. Acta 646:365-7
Abstract	L-Sorbose is phosphorylated by Escherichia coli by two distinct Enzymes II of the phosphoenolpyruvate-dependent phosphotransferase system. The glucose Enzyme II (specified by the gene ptsG) phosphorylates L-sorbose with an apparent Km of 0.08 +/- 0.03 mM and V of 31.8 +/- 3.5 nmol . mg-1 . min-1 whilst the fructose Enzyme II (specified by the gene ptsF) phosphorylates it with an apparent Km of 28.9 +/- 2.7 mM and V of 20.2 +/- 0.8 nmol . mg-1 . min-1. L-Sorbose induces neither of these Enzymes II, but sorbose inhibits the growth of strains expressing either of these functions constitutively. Mutants that have lost their sensitivity to L-sorbose are found to have lost either the glucose or the fructose phosphotransferase Enzyme II.U
Links	PubMed
Keywords	Escherichia coli; Genotype; Kinetics; Phenotype; Phosphoenolpyruvate Sugar Phosphotransferase System; Phosphotransferases (Nitrogenous Group Acceptor); Sorbose; Species Specificity
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Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: AS 1 NCBI Taxon ID: 83333	Genotype of Reference Strain: ptsF Genotype of Experimental Strain : ptsF -	Reference Condition:					Resistant to sorbose, table 1
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: HK737 NCBI Taxon ID: 83333	Genotype of Reference Strain: ptsG Genotype of Experimental Strain : ptsG -	Reference Condition:					Mutant is resistant to sorbose, table 1
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