Difference between revisions of "PMID:4868365"

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{|  id="Z4fa987fb1d7e3"  class=" tableEdit PMID_info_table" 
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!align=left  |Citation
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'''Kaplan, S and Anderson, D'''  (1968) Selection of temperature-sensitive activating enzyme mutants in Escherichia coli.''J. Bacteriol.'' '''95''':991-7
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!align=left  |Abstract
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A method based on temperature-conditional resistance to thymineless death has been designed to facilitate the isolation of amino acid-activating enzyme mutants. This method may be modified to obtain a greater or lesser proportion of activating enzyme mutants. In the latter instance, an increased proportion of temperature-sensitive macromolecule mutants of other types is obtained. Additional uses of this procedure are discussed.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=4868365 PubMed]
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!align=left  |Keywords
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Amino Acids; Bacterial Proteins; Carbon Isotopes; Escherichia coli; Ligases; Molecular Biology; Mutation; Proline; RNA, Bacterial; Temperature; Thymine; Tritium; Uracil
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==Main Points of the Paper ==
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== Materials and Methods Used ==
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==Phenotype Annotations==
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==Notes==
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==References==
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[[Category:Publication]]

Revision as of 15:54, 8 May 2012

Citation

Kaplan, S and Anderson, D (1968) Selection of temperature-sensitive activating enzyme mutants in Escherichia coli.J. Bacteriol. 95:991-7

Abstract

A method based on temperature-conditional resistance to thymineless death has been designed to facilitate the isolation of amino acid-activating enzyme mutants. This method may be modified to obtain a greater or lesser proportion of activating enzyme mutants. In the latter instance, an increased proportion of temperature-sensitive macromolecule mutants of other types is obtained. Additional uses of this procedure are discussed.

Links

PubMed

Keywords

Amino Acids; Bacterial Proteins; Carbon Isotopes; Escherichia coli; Ligases; Molecular Biology; Mutation; Proline; RNA, Bacterial; Temperature; Thymine; Tritium; Uracil

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

</protect>

Notes

References

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