PMID:4868365

Citation	Kaplan, S and Anderson, D (1968) Selection of temperature-sensitive activating enzyme mutants in Escherichia coli.J. Bacteriol. 95:991-7
Abstract	A method based on temperature-conditional resistance to thymineless death has been designed to facilitate the isolation of amino acid-activating enzyme mutants. This method may be modified to obtain a greater or lesser proportion of activating enzyme mutants. In the latter instance, an increased proportion of temperature-sensitive macromolecule mutants of other types is obtained. Additional uses of this procedure are discussed.
Links	PubMed
Keywords	Amino Acids; Bacterial Proteins; Carbon Isotopes; Escherichia coli; Ligases; Molecular Biology; Mutation; Proline; RNA, Bacterial; Temperature; Thymine; Tritium; Uracil
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Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: 6C5 NCBI Taxon ID: 83333	Genotype of Reference Strain: pheS Genotype of Experimental Strain : pheS	Reference Condition: 28C Experimental Condition: 42C			ECO:0000182	in vitro culture assay data	Thymineless death resistance in thyA. Under the condition of 42C.
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