Difference between revisions of "PMID:363701"

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{|  id="W4fb5641a51e80"  class=" tableEdit PMID_info_table" 
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!align=left  |Citation
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'''Dabbs, ER'''  (1978) Kasugamycin-dependent mutants of Escherichia coli.''J. Bacteriol.'' '''136''':994-1001
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!align=left  |Abstract
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Kasugamycin-dependent mutants have been isolated from Escherichia coli B. They were obtained through mutagenesis with ethyl methane sulfonate or nitrosoguanidine in conjunction with an antibiotic underlay technique. In the case of nitrosoguanidine, dependent mutants were obtained at a frequency of about 3% of survivors growing up in the selection. In the case of ethyl methane sulfonate, the corresponding value was 1%. Nineteen mutants showing a kasugamycin-dependent phenotype were studied. In terms of response to various temperatures and antibiotic concentrations, they were very heterogeneous, although most fell into two general classes. Genetic analysis indicated that in at least some cases, the kasugamycin-dependent phenotype was the product of two mutations. Two-dimensional gel electropherograms revealed alterations in the ribosomal proteins of seven mutants. One mutant had an alteration in protein S13, and one had an alteration in protein L14. Three showed changes in protein S9. Each of two mutants had changes in two proteins, S18 and L11. Three of these mutants additionally had protein S18 occurring in a partly altered, partly unaltered form.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=363701 PubMed]
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!align=left  |Keywords
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Aminoglycosides; Anti-Bacterial Agents; Bacterial Proteins; Chromosome Mapping; Chromosomes, Bacterial; Dose-Response Relationship, Drug; Escherichia coli; Genes; Mutation; Ribosomal Proteins; Temperature
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==Main Points of the Paper ==
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{{LitSignificance}}
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== Materials and Methods Used ==
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==Phenotype Annotations==
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==Notes==
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==References==
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[[Category:Publication]]

Revision as of 15:48, 17 May 2012

Citation

Dabbs, ER (1978) Kasugamycin-dependent mutants of Escherichia coli.J. Bacteriol. 136:994-1001

Abstract

Kasugamycin-dependent mutants have been isolated from Escherichia coli B. They were obtained through mutagenesis with ethyl methane sulfonate or nitrosoguanidine in conjunction with an antibiotic underlay technique. In the case of nitrosoguanidine, dependent mutants were obtained at a frequency of about 3% of survivors growing up in the selection. In the case of ethyl methane sulfonate, the corresponding value was 1%. Nineteen mutants showing a kasugamycin-dependent phenotype were studied. In terms of response to various temperatures and antibiotic concentrations, they were very heterogeneous, although most fell into two general classes. Genetic analysis indicated that in at least some cases, the kasugamycin-dependent phenotype was the product of two mutations. Two-dimensional gel electropherograms revealed alterations in the ribosomal proteins of seven mutants. One mutant had an alteration in protein S13, and one had an alteration in protein L14. Three showed changes in protein S9. Each of two mutants had changes in two proteins, S18 and L11. Three of these mutants additionally had protein S18 occurring in a partly altered, partly unaltered form.

Links

PubMed

Keywords

Aminoglycosides; Anti-Bacterial Agents; Bacterial Proteins; Chromosome Mapping; Chromosomes, Bacterial; Dose-Response Relationship, Drug; Escherichia coli; Genes; Mutation; Ribosomal Proteins; Temperature

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Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status
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Notes

References

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