PMID:3918006

Citation	Sullivan, MA and Bock, RM (1985) Isolation and characterization of antisuppressor mutations in Escherichia coli.J. Bacteriol. 161:377-84
Abstract	Nonsense mutations in lacI have been shown to be useful as indicators of the efficiency of nonsense suppression. From strains containing supE and a lacI nonsense mutation, selection for LacI- mutants has resulted in the isolation of four antisuppressor mutations. Tn10 insertions linked to these mutations were isolated and used to group the four mutations into three loci. The asuA1 and asuA2 mutations are linked to trp, reduce suppression by supE approximately twofold, and affect a variety of suppressors. The asuB3 mutation was mapped by P1 cotransduction to rpsL but does not confer resistance to streptomycin. The asuC4 mutation reduced suppression by supE by 95% and was shown biochemically to result in the loss of two pseudouridine modifications from the 3' side of the anticodon stem and loop of tRNA2Gln. This mutation is linked to purF, suggesting that it is a new allele of hisT.
Links	PubMed
Keywords	Base Sequence; Codon; Escherichia coli; Genes, Bacterial; Lac Operon; Mutation; Pseudouridine; RNA, Transfer; RNA, Transfer, Amino Acyl; Suppression, Genetic; Transduction, Genetic; beta-Galactosidase
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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: MK60E/I3116 NCBI Taxon ID: 83333	Genotype of Reference Strain: glnV44 rpsL+ Genotype of Experimental Strain : glnV44 rpsL-asuB3	Reference Condition:			0000049	reporter gene assay	The efficiency of suppression was measured and there was seen to be a significant decrease as seen in table 5.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: MK60E/I3116 NCBI Taxon ID: 83333	Genotype of Reference Strain: glnV44 purF+ Genotype of Experimental Strain : glnV44 purF-asuC4	Reference Condition:			0000049	reporter gene assay	The efficiency of suppression was measured and there was seen to be a significant decrease as seen in table 5.
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