Difference between revisions of "PMID:342516"

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{|  id="A4fce4c09a6f2a"  class=" tableEdit PMID_info_table" 
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!align=left  |Citation
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'''Long, WS, Slayman, CL and Low, KB'''  (1978) Production of giant cells of Escherichia coli.''J. Bacteriol.'' '''133''':995-1007
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!align=left  |Abstract
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Giant cells, with volumes up to 500-fold those of normal cells, have been produced by both genetic and pharmacological means in Escherichia coli K-12. In the genetic approach, an envB or mon mutation (conferring rounded or irregular morphology) was combined with a lon mutation (block of septation after irradiation). UV irradiation and subsequent incubation for 2 to 5 h in a rich medium supplemented with 1% sodium chloride led t; production of polymorphic giant cells. In the pharmacological approach, incubation of several different strains of E. coli K-12 with the drug 6-amidinopenicillanic acid (FL1060) in the same rich medium gave rise to a homogeneous population of smoothly rounded giant cells.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=342516 PubMed]
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!align=left  |Keywords
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Cell Division; Escherichia coli; Microscopy, Phase-Contrast; Mutation; Phenotype; Transduction, Genetic; Ultraviolet Rays
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==Main Points of the Paper ==
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{{LitSignificance}}
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== Materials and Methods Used ==
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{{LitMaterials}}
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==Phenotype Annotations==
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==Notes==
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==References==
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[[Category:Publication]]

Revision as of 13:12, 5 June 2012

Citation

Long, WS, Slayman, CL and Low, KB (1978) Production of giant cells of Escherichia coli.J. Bacteriol. 133:995-1007

Abstract

Giant cells, with volumes up to 500-fold those of normal cells, have been produced by both genetic and pharmacological means in Escherichia coli K-12. In the genetic approach, an envB or mon mutation (conferring rounded or irregular morphology) was combined with a lon mutation (block of septation after irradiation). UV irradiation and subsequent incubation for 2 to 5 h in a rich medium supplemented with 1% sodium chloride led t; production of polymorphic giant cells. In the pharmacological approach, incubation of several different strains of E. coli K-12 with the drug 6-amidinopenicillanic acid (FL1060) in the same rich medium gave rise to a homogeneous population of smoothly rounded giant cells.

Links

PubMed

Keywords

Cell Division; Escherichia coli; Microscopy, Phase-Contrast; Mutation; Phenotype; Transduction, Genetic; Ultraviolet Rays

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

</protect>

Notes

References

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