Difference between revisions of "PMID:342516"
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increased cell size | increased cell size | ||
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| − | + | 0000181 | |
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| − | + | in vitro assay data | |
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| − | The cross of two mutant strains, D23 | + | The cross of two mutant strains, D23 and 3.00-M6 confer the mutation that produce giant cells by combining mutations that inhibit septation and changes cells to rounded or amorphous shape. both strains are from previous work. |
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| + | |- | ||
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| + | a mutation or genetic difference within a strain | ||
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| + | *Taxon: Escherichia coli | ||
| + | *Strain: K-12 | ||
| + | *Substrain: LL81 | ||
| + | *NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333] | ||
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| + | *Genotype of Reference Strain: ''ampA<sup>+</sup> envB<sup>+</sup> sloB<sup>+</sup> lon<sup>+</sup> | ||
| + | *Genotype of Experimental Strain : ''ampA1 envB1 sloB1 lon-10'' | ||
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| + | *Reference Condition: | ||
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| + | 0000041 | ||
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| + | increased cell size | ||
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| + | 0000181 | ||
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| + | in vitro assay data | ||
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| + | The cross of two mutant strains, D23 and 3.00-M6 confer the mutation that produce giant cells by combining mutations that inhibit septation and changes cells to rounded or amorphous shape. Irradiation causes heterogeneity in cell shape. See figure 1 for visualization 5 hours after irradiation. | ||
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| − | [[Category:Publication]] | + | [[Category:Publication]][[Category:Papers referenced in the LaRossa chapter]] [[Category:LaRossa citations that need review]] |
Latest revision as of 11:10, 12 July 2012
| Citation |
Long, WS, Slayman, CL and Low, KB (1978) Production of giant cells of Escherichia coli.J. Bacteriol. 133:995-1007 |
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| Abstract |
Giant cells, with volumes up to 500-fold those of normal cells, have been produced by both genetic and pharmacological means in Escherichia coli K-12. In the genetic approach, an envB or mon mutation (conferring rounded or irregular morphology) was combined with a lon mutation (block of septation after irradiation). UV irradiation and subsequent incubation for 2 to 5 h in a rich medium supplemented with 1% sodium chloride led t; production of polymorphic giant cells. In the pharmacological approach, incubation of several different strains of E. coli K-12 with the drug 6-amidinopenicillanic acid (FL1060) in the same rich medium gave rise to a homogeneous population of smoothly rounded giant cells. |
| Links | |
| Keywords |
Cell Division; Escherichia coli; Microscopy, Phase-Contrast; Mutation; Phenotype; Transduction, Genetic; Ultraviolet Rays |
| edit table |
Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
See Help:AnnotationTable for details on how to edit this table.
<protect>
| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
|
a mutation or genetic difference within a strain |
|
|
|
0000041 |
increased cell size |
0000181 |
in vitro assay data |
The cross of two mutant strains, D23 and 3.00-M6 confer the mutation that produce giant cells by combining mutations that inhibit septation and changes cells to rounded or amorphous shape. both strains are from previous work. |
|
|
a mutation or genetic difference within a strain |
|
|
|
0000041 |
increased cell size |
0000181 |
in vitro assay data |
The cross of two mutant strains, D23 and 3.00-M6 confer the mutation that produce giant cells by combining mutations that inhibit septation and changes cells to rounded or amorphous shape. Irradiation causes heterogeneity in cell shape. See figure 1 for visualization 5 hours after irradiation. |
|
| edit table |
</protect>
Notes
References
See Help:References for how to manage references in omp dev.
