PMID:374348

Citation	Baughman, GA and Fahnestock, SR (1979) Chloramphenicol resistance mutation in Escherichia coli which maps in the major ribosomal protein gene cluster.J. Bacteriol. 137:1315-23
Abstract	Localized mutagenesis and selection for streptomycin resistance were utilized to isolate a chloramphenicol resistance mutation in Escherichia coli K-12 linked to the strA (rpsL) locus. Bacteriophage P1 transduction verified the map position of the new resistance mutation at 72 min, placing it within a dense cluster of ribosomal protein genes. The map position differs from that of known cmlA and cmlB mutations, which map at 18 and 21 min, respectively. Ribosomes prepared from chloramphenicol-resistant and -sensitive isogenic transductants were analyzed in vitro for activity in formation of N-formylmethionyl-puromycin, polyphenylalanine, and polylysine in the presence of inhibitory concentrations of chloramphenicol. Comparisons were also made of 14C-chloramphenicol binding to 70S ribosomes and of the two-dimensional polyacrylamide gel electrophoresis pattern of ribosomal proteins from each strain. There was no detectable difference between ribosomes from sensitive and resistant strains as measured by these assays. Enzymatic modification by chloramphenicol acetyltransferase is not responsible for the observed phenotype.
Links	PubMed
Keywords	Chloramphenicol; Chromosome Mapping; Drug Resistance, Microbial; Escherichia coli; Genes; Mutation; Ribosomal Proteins; Streptomycin; Transduction, Genetic
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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: RE1 NCBI Taxon ID: 83333	Genotype of Reference Strain: cmlA Genotype of Experimental Strain : cmlA1	Reference Condition:					Resistant to choramphenicol as measured by zones of growth inhibition.
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