Difference between revisions of "PMID:52916"

From omp dev
Jump to: navigation, search
(New PMID: Page!)
 
(Fill PMID: Page!)
 
Line 1: Line 1:
 +
{{RightTOC}}
  
 +
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.3325.J5053ad9002aea-->
 +
<!--
 +
******************************************************************************************
 +
*
 +
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
 +
*
 +
****************************************************************************************** -->
 +
{|  id="J5053ad9002aea"  class=" tableEdit PMID_info_table" 
 +
 +
|-
 +
!align=left  |Citation
 +
||
 +
'''Adams, E '''  (1975) Studies in gram staining. ''Stain Technol'' '''50''':227-31
 +
|-
 +
!align=left  |Abstract
 +
||
 +
Gram-negative bacteria stained with crystal violet are decolorized by 95% alcohol within 2 min, whereas Gram-positive bacteria require at least 3 min treatment. Aqueous solutions of safranin, neutral red, and fuschsin replace crystal violet from stained Gram-positive bacteria more quickly than alcohol alone, and alcoholic solutions of these counterstains are in most cases still more effective. Treatment of crystal violet-stained organisms with alcoholic safranin (0.25%) for 15 sec will distinguish Gram-positive bacteria (violet) from Gram-negative bacteria (pink). Alcohol containing very low concentrations of iodine generally decolorizes crystal violet-stained Gram-positive bacteria more quickly than alcohol alone. Increasing concentrations of iodine in alcohol reduce the rate of decolorization of stained bacteria, but stained Gram-negative bacteria are still readily decolorized. The addition of 0.1% iodine to alcohol increases the rate of extraction of crystal violet by alcohol from Gram-negative organisms, but delays extraction of dye from Gram-positive organisms, and this applies when counterstain is also present. A two-solution modification of Gram staining is described in which crystal violet-stained bacteria are treated with an alcoholic solution of safranin, fuchsin, and iodine.
 +
|-
 +
!align=left  |Links
 +
||
 +
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=52916 PubMed]
 +
 +
|-
 +
!align=left  |Keywords
 +
||
 +
Bacteria; Color; Coloring Agents; Gentian Violet; Iodine; Rosaniline Dyes; Staining and Labeling
 +
 +
|- class="tableEdit_footer"
 +
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.3325.J5053ad9002aea&page=3325&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table]</span> ||
 +
|}
 +
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.3325.J5053ad9002aea-->
 +
 +
==Main Points of the Paper ==
 +
{{LitSignificance}}
 +
 +
== Materials and Methods Used ==
 +
{{LitMaterials}}
 +
 +
==Phenotype Annotations==
 +
{{AnnotationTableHelp}}
 +
<protect><!--box uid=d41d8cd98f00b204e9800998ecf8427e.3325.H5053ad9008beb-->
 +
<!--
 +
******************************************************************************************
 +
*
 +
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
 +
*
 +
****************************************************************************************** -->
 +
{|  id="H5053ad9008beb"  class=" tableEdit Phenotype_Table_2" 
 +
|-
 +
!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
 +
 +
|- class="tableEdit_footer"
 +
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.3325.H5053ad9008beb&page=3325&pagename={{FULLPAGENAMEE}}&type=0&template=Phenotype_Table_2 edit table]</span> || || || || || || || || ||
 +
|}
 +
<!--box uid=d41d8cd98f00b204e9800998ecf8427e.3325.H5053ad9008beb--></protect>
 +
 +
==Notes==
 +
 +
==References==
 +
{{RefHelp}}
 +
<references/>
 +
 +
 +
[[Category:Publication]]

Latest revision as of 17:20, 14 September 2012

Citation

Adams, E (1975) Studies in gram staining. Stain Technol 50:227-31

Abstract

Gram-negative bacteria stained with crystal violet are decolorized by 95% alcohol within 2 min, whereas Gram-positive bacteria require at least 3 min treatment. Aqueous solutions of safranin, neutral red, and fuschsin replace crystal violet from stained Gram-positive bacteria more quickly than alcohol alone, and alcoholic solutions of these counterstains are in most cases still more effective. Treatment of crystal violet-stained organisms with alcoholic safranin (0.25%) for 15 sec will distinguish Gram-positive bacteria (violet) from Gram-negative bacteria (pink). Alcohol containing very low concentrations of iodine generally decolorizes crystal violet-stained Gram-positive bacteria more quickly than alcohol alone. Increasing concentrations of iodine in alcohol reduce the rate of decolorization of stained bacteria, but stained Gram-negative bacteria are still readily decolorized. The addition of 0.1% iodine to alcohol increases the rate of extraction of crystal violet by alcohol from Gram-negative organisms, but delays extraction of dye from Gram-positive organisms, and this applies when counterstain is also present. A two-solution modification of Gram staining is described in which crystal violet-stained bacteria are treated with an alcoholic solution of safranin, fuchsin, and iodine.

Links

PubMed

Keywords

Bacteria; Color; Coloring Agents; Gentian Violet; Iodine; Rosaniline Dyes; Staining and Labeling

edit table

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status
edit table

</protect>

Notes

References

See Help:References for how to manage references in omp dev.

Retrieved from "http://bcbp-ftq4xm2.biobio.tamu.edu/omp/dev/index.php?title=PMID:52916&oldid=8063"