Difference between revisions of "PMID:14009486"
(Fill PMID: Page!) |
(→Main Points of the Paper) |
||
| Line 35: | Line 35: | ||
==Main Points of the Paper == | ==Main Points of the Paper == | ||
{{LitSignificance}} | {{LitSignificance}} | ||
| + | *Thiogalactoside transacetylase is identified | ||
| + | *Characterization of the purified protein is also described | ||
| + | **rate of formation of acetyl-IPTG as a function of time- reaction is linear | ||
| + | **optimal pH is 7.0 in phosphate buffer, but 8.2 in Tris buffer | ||
| + | **KM<4x10-5 M for acetyl-CoA | ||
| + | **Butyryl-CoA was about 10% as effective as acetyl-CoA as donors | ||
| + | **catalyzes the transfer of acetyl from acetyl-CoA to thiogalactosides | ||
| + | **divalent cations are not required for the formation of acetyl-IPTG | ||
| + | **activity is slightly inhibited by 0.001 M p-Chloromercuribenzoate (31% inhibition) | ||
| + | **essentially all the activity present in the supernatant fraction | ||
== Materials and Methods Used == | == Materials and Methods Used == | ||
Revision as of 17:10, 17 September 2012
| Citation |
ZABIN, I , KEPES, A and MONOD, J (1962) Thiogalactoside transacetylase. J. Biol. Chem. 237:253-7 |
|---|---|
| Abstract |
No abstract in PubMed |
| Links | |
| Keywords |
Transferases/chemistry |
| edit table |
Main Points of the Paper
Please summarize the main points of the paper.
- Thiogalactoside transacetylase is identified
- Characterization of the purified protein is also described
- rate of formation of acetyl-IPTG as a function of time- reaction is linear
- optimal pH is 7.0 in phosphate buffer, but 8.2 in Tris buffer
- KM<4x10-5 M for acetyl-CoA
- Butyryl-CoA was about 10% as effective as acetyl-CoA as donors
- catalyzes the transfer of acetyl from acetyl-CoA to thiogalactosides
- divalent cations are not required for the formation of acetyl-IPTG
- activity is slightly inhibited by 0.001 M p-Chloromercuribenzoate (31% inhibition)
- essentially all the activity present in the supernatant fraction
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
See Help:AnnotationTable for details on how to edit this table.
<protect>
| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
| edit table |
</protect>
Notes
References
See Help:References for how to manage references in omp dev.
