Difference between revisions of "PMID:6365956"

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'''Trepeta, RW  and Edberg, SC '''  (1984) Methylumbelliferyl-beta-D-glucuronide-based medium for rapid isolation and identification of Escherichia coli. ''J. Clin. Microbiol.'' '''19''':172-4
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!align=left  |Abstract
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Escherichia coli is the most common gram-negative microbe isolated and identified in clinical microbiology laboratories. It can be identified within 1 h by oxidase, indole, lactose, and beta-glucuronidase tests. The oxidase and indole tests are performed as spot tests, and lactose fermentation is read directly from MacConkey agar. It was found that 4-methylumbelliferyl-beta-D-glucuronide could be incorporated directly into a modified MacConkey agar to directly detect the presence of beta-glucuronidase. Other characteristics of MacConkey agar were not affected. The incorporation of 4-methylumbelliferyl-beta-D-glucuronide into modified agar obviated the need for manufacture, quality control, and incubation of reagent-containing test tubes. The time needed to identify E. coli strains was reduced from 1 h to 5 min, and the ability to detect this species in mixed specimens was also enhanced.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6365956 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC271011 PMC271011]
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Agar; Culture Media; Escherichia coli/classification; Escherichia coli/enzymology; Escherichia coli/isolation & purification; Escherichia coli/metabolism; Fermentation; Fluorescence; Glucuronates/metabolism; Glucuronidase/metabolism; Hymecromone/analogs & derivatives; Hymecromone/metabolism; Indoles/metabolism; Lactose/metabolism; Oxidoreductases/metabolism; Time Factors
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==Main Points of the Paper ==
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== Materials and Methods Used ==
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==Phenotype Annotations==
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==Notes==
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==References==
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[[Category:Publication]]

Revision as of 17:43, 18 September 2012

Citation

Trepeta, RW and Edberg, SC (1984) Methylumbelliferyl-beta-D-glucuronide-based medium for rapid isolation and identification of Escherichia coli. J. Clin. Microbiol. 19:172-4

Abstract

Escherichia coli is the most common gram-negative microbe isolated and identified in clinical microbiology laboratories. It can be identified within 1 h by oxidase, indole, lactose, and beta-glucuronidase tests. The oxidase and indole tests are performed as spot tests, and lactose fermentation is read directly from MacConkey agar. It was found that 4-methylumbelliferyl-beta-D-glucuronide could be incorporated directly into a modified MacConkey agar to directly detect the presence of beta-glucuronidase. Other characteristics of MacConkey agar were not affected. The incorporation of 4-methylumbelliferyl-beta-D-glucuronide into modified agar obviated the need for manufacture, quality control, and incubation of reagent-containing test tubes. The time needed to identify E. coli strains was reduced from 1 h to 5 min, and the ability to detect this species in mixed specimens was also enhanced.

Links

PubMed PMC271011

Keywords

Agar; Culture Media; Escherichia coli/classification; Escherichia coli/enzymology; Escherichia coli/isolation & purification; Escherichia coli/metabolism; Fermentation; Fluorescence; Glucuronates/metabolism; Glucuronidase/metabolism; Hymecromone/analogs & derivatives; Hymecromone/metabolism; Indoles/metabolism; Lactose/metabolism; Oxidoreductases/metabolism; Time Factors

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Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status
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Notes

References

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