Difference between revisions of "PMID:6247616"

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{|  id="T4fe894c4c369e"  class=" tableEdit PMID_info_table" 
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!align=left  |Citation
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'''Danchin, A and Dondon, L'''  (1980) Serine sensitivity of Escherichia coli K 12: partial characterization of a serine resistnat mutant that is extremely sensitive to 2-ketobutyrate.''Mol. Gen. Genet.'' '''178''':155-64
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!align=left  |Abstract
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E. coli wild type bacteria display sensitivity towards serine. A selection medium is described which allows selection of serine resistant mutants. One such mutant is described which presents pleiotropic alterations: it exhibits a thermosensitive growth pattern, alteration in the metabolism of the pppGpp and ppGpp nucleotides, cAMP intracellular level alteration, extreme sensitivity to 2-ketobutyric acid and a defect in the phosphotransferases permeation system. A conjecture explaining these apparently unrelated defects supposes that serine metabolism interferes via phosphoenol pyruvate with a cytoplasmic control of membrane activity (the mutant would be defective in the coupling between membrane and the protein responsible for its cytoplasmic control) and that 2-ketobutyrate is an effector of this activity.
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!align=left  |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6247616 PubMed]
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!align=left  |Keywords
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Butyrates; Butyric Acids; Culture Media; Cyclic AMP; Drug Resistance, Microbial; Escherichia coli; Guanosine Pentaphosphate; Guanosine Tetraphosphate; Keto Acids; Mutation; Phenotype; Serine
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==Main Points of the Paper ==
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{{LitSignificance}}
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== Materials and Methods Used ==
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{{LitMaterials}}
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==Phenotype Annotations==
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{{AnnotationTableHelp}}
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="P4fe894c4d36f7"  class=" tableEdit Phenotype_Table_2" 
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!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: FB8
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*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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*Genotype of Reference Strain: ''sbA<sup>+</sup>''
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*Genotype of Experimental Strain : ''SDT1 sbaA<sup>-</sup>''
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*Reference Condition: 33<sup>o</sup>C
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*Experimental Condition: 43<sup>o</sup>C
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0000271
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temperature-dependent growth
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0000181
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in vitro assay data
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Decrease in RNA synthesis at high temperature. See figure a for 2B for gel electrophoresis results. 
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: FB8
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*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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*Genotype of Reference Strain: ''sbA<sup>+</sup>''
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*Genotype of Experimental Strain : ''SDT1 sbaA<sup>-</sup>''
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*Reference Condition:
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0000252
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metabolite accumulation
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0000181
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in vitro assay data
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Decreased metabolism of ppGpp compared to the parent strain. See figure 3 a & b for full results.
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|-
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a mutation or genetic difference within a strain
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|
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: FB8
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*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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*Genotype of Reference Strain: ''sbA<sup>+</sup>''
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*Genotype of Experimental Strain : ''SDT1 sbaA<sup>-</sup>''
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|
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*Reference Condition:
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0000277
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decreased beta-galactosidase activity
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|
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0000181
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in vitro assay data
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|
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Significant decrease in beta-galactosidase activity compared to the parent strain.  See Figure 4 for full results.
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|
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|-
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|
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a mutation or genetic difference within a strain
 +
|
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*Taxon: Escherichia coli
 +
*Strain: K-12
 +
*Substrain: FB8
 +
*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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|
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*Genotype of Reference Strain: ''sbA<sup>+</sup>''
 +
*Genotype of Experimental Strain : ''SDT1 sbaA<sup>-</sup>''
 +
|
 +
*Reference Condition:
 +
|
 +
0000278
 +
|
 +
increased beta-galactosidase activity
 +
|
 +
0000181
 +
|
 +
in vitro assay data
 +
|
 +
the introduction of 1mM cAMP increased beta-galactosidase activity but less than the parent strain.  See Figure 4 for the full experimental results.
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|
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|- class="tableEdit_footer"
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|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.3221.P4fe894c4d36f7&page=3221&pagename={{FULLPAGENAMEE}}&type=0&template=Phenotype_Table_2 edit table]</span> || || || || || || || || ||
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==Notes==
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*terms place holders
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==References==
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{{RefHelp}}
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<references/>
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[[Category:Publication]] [[Category:Papers referenced in the LaRossa chapter]]
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[[Category:Papers referenced in the LaRossa chapter]]

Latest revision as of 18:22, 22 May 2013

Citation

Danchin, A and Dondon, L (1980) Serine sensitivity of Escherichia coli K 12: partial characterization of a serine resistnat mutant that is extremely sensitive to 2-ketobutyrate.Mol. Gen. Genet. 178:155-64

Abstract

E. coli wild type bacteria display sensitivity towards serine. A selection medium is described which allows selection of serine resistant mutants. One such mutant is described which presents pleiotropic alterations: it exhibits a thermosensitive growth pattern, alteration in the metabolism of the pppGpp and ppGpp nucleotides, cAMP intracellular level alteration, extreme sensitivity to 2-ketobutyric acid and a defect in the phosphotransferases permeation system. A conjecture explaining these apparently unrelated defects supposes that serine metabolism interferes via phosphoenol pyruvate with a cytoplasmic control of membrane activity (the mutant would be defective in the coupling between membrane and the protein responsible for its cytoplasmic control) and that 2-ketobutyrate is an effector of this activity.

Links

PubMed

Keywords

Butyrates; Butyric Acids; Culture Media; Cyclic AMP; Drug Resistance, Microbial; Escherichia coli; Guanosine Pentaphosphate; Guanosine Tetraphosphate; Keto Acids; Mutation; Phenotype; Serine

edit table

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: FB8
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: sbA+
  • Genotype of Experimental Strain : SDT1 sbaA-
  • Reference Condition: 33oC
  • Experimental Condition: 43oC

0000271

temperature-dependent growth

0000181

in vitro assay data

Decrease in RNA synthesis at high temperature. See figure a for 2B for gel electrophoresis results.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: FB8
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: sbA+
  • Genotype of Experimental Strain : SDT1 sbaA-
  • Reference Condition:

0000252

metabolite accumulation

0000181

in vitro assay data

Decreased metabolism of ppGpp compared to the parent strain. See figure 3 a & b for full results.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: FB8
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: sbA+
  • Genotype of Experimental Strain : SDT1 sbaA-
  • Reference Condition:

0000277

decreased beta-galactosidase activity

0000181

in vitro assay data

Significant decrease in beta-galactosidase activity compared to the parent strain. See Figure 4 for full results.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: FB8
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: sbA+
  • Genotype of Experimental Strain : SDT1 sbaA-
  • Reference Condition:

0000278

increased beta-galactosidase activity

0000181

in vitro assay data

the introduction of 1mM cAMP increased beta-galactosidase activity but less than the parent strain. See Figure 4 for the full experimental results.


edit table

</protect>

Notes

  • terms place holders

References

See Help:References for how to manage references in omp dev.

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