Difference between revisions of "PMID:6802799"

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{|  id="Q4ff364aa0ffb1"  class=" tableEdit PMID_info_table" 
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'''Newman, EB, Malik, N and Walker, C'''  (1982) L-serine degradation in Escherichia coli K-12: directly isolated ssd mutants and their intragenic revertants.''J. Bacteriol.'' '''150''':710-5
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!align=left  |Abstract
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Two methods for the direct isolation of spontaneous ssd mutants of Escherichia coli K-12 strains are described; (i) by growth with L-serine as the carbon source, and (ii) by low-level kanamycin resistance. A newly isolated mutant had the same phenotype as the mutant described previously, including inefficient use of the glucose, inability to growth with succinate, altered transport characteristics, and altered resistance to certain growth effectors. Succinate-utilizing derivatives which appear to be intragenic are characterized in detail. The relation between the mutants isolated here and mutants which are thought to have impairment in a system of coupling respiratory energy to active transport (ecfB mutants) is discussed.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6802799 PubMed]
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!align=left  |Keywords
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Biological Transport, Active; Drug Resistance, Microbial; Escherichia coli; Genes, Bacterial; Glucose; Kanamycin; Mutation; Serine; Succinates; Succinic Acid
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==Main Points of the Paper ==
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{{LitSignificance}}
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== Materials and Methods Used ==
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{{LitMaterials}}
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==Phenotype Annotations==
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{{AnnotationTableHelp}}
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="K4ff364aa13aac"  class=" tableEdit Phenotype_Table_2" 
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!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: CU1008
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*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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*Genotype of Reference Strain: ''ecfB''
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*Genotype of Experimental Strain : ''ecfB'' in strains KEC1 - KEC20
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*Reference Condition:
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OMP:0000266
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utilizes carbon-containing compound
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Carbon utilization of L-Serine. See table 1
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: W4977
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*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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*Genotype of Reference Strain: ''ecfB''
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*Genotype of Experimental Strain : ''ecfB'' in strains KEC21 - KEC30
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*Reference Condition:
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OMP:0000266
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utilizes carbon-containing compound
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Carbon utilization of L-Serine. See table 1
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: CU1008
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*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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*Genotype of Reference Strain: ''ssd''
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*Genotype of Experimental Strain : ''ssd' in mutant strain KEC3
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*Reference Condition:
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OMP:0000276
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increased resistance to antimicrobial compound
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Resistance to Kanamycin
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: CU1008
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*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=83333&lvl=3&lin=f&keep=1&srchmode=1&unlock 83333]
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*Genotype of Reference Strain: ''ssd''
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*Genotype of Experimental Strain : ''ssd' in mutant strain KEC3
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*Reference Condition:
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OMP:0000276
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increased resistance to antimicrobial compound
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Resistance to Neomycin
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==Notes==
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*L-serine phenotype:
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**ECOID: This is a solid medium assay in minimal medium.
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*Nemycin/kanamycin: It is an aminoglcoside and term has been requested.
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==References==
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{{RefHelp}}
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<references/>
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[[Category:Publication]] [[Category:Papers referenced in the LaRossa chapter]] [[Category:LaRossa citations that need review]]

Latest revision as of 17:53, 23 January 2013

Citation

Newman, EB, Malik, N and Walker, C (1982) L-serine degradation in Escherichia coli K-12: directly isolated ssd mutants and their intragenic revertants.J. Bacteriol. 150:710-5

Abstract

Two methods for the direct isolation of spontaneous ssd mutants of Escherichia coli K-12 strains are described; (i) by growth with L-serine as the carbon source, and (ii) by low-level kanamycin resistance. A newly isolated mutant had the same phenotype as the mutant described previously, including inefficient use of the glucose, inability to growth with succinate, altered transport characteristics, and altered resistance to certain growth effectors. Succinate-utilizing derivatives which appear to be intragenic are characterized in detail. The relation between the mutants isolated here and mutants which are thought to have impairment in a system of coupling respiratory energy to active transport (ecfB mutants) is discussed.

Links

PubMed

Keywords

Biological Transport, Active; Drug Resistance, Microbial; Escherichia coli; Genes, Bacterial; Glucose; Kanamycin; Mutation; Serine; Succinates; Succinic Acid

edit table

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: CU1008
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: ecfB
  • Genotype of Experimental Strain : ecfB in strains KEC1 - KEC20
  • Reference Condition:

OMP:0000266

utilizes carbon-containing compound

Carbon utilization of L-Serine. See table 1

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: W4977
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: ecfB
  • Genotype of Experimental Strain : ecfB in strains KEC21 - KEC30
  • Reference Condition:

OMP:0000266

utilizes carbon-containing compound

Carbon utilization of L-Serine. See table 1

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: CU1008
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: ssd
  • Genotype of Experimental Strain : ssd' in mutant strain KEC3
  • Reference Condition:

OMP:0000276

increased resistance to antimicrobial compound

Resistance to Kanamycin

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: CU1008
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: ssd
  • Genotype of Experimental Strain : ssd' in mutant strain KEC3
  • Reference Condition:

OMP:0000276

increased resistance to antimicrobial compound

Resistance to Neomycin


edit table

</protect>

Notes

  • L-serine phenotype:
    • ECOID: This is a solid medium assay in minimal medium.
  • Nemycin/kanamycin: It is an aminoglcoside and term has been requested.

References

See Help:References for how to manage references in omp dev.

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