Difference between revisions of "PMID:8376351"

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{|  id="M4ffefc6b6ce5b"  class=" tableEdit PMID_info_table" 
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!align=left  |Citation
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'''Summers, WC and Raksin, P'''  (1993) A method for selection of mutations at the tdk locus in Escherichia coli.''J. Bacteriol.'' '''175''':6049-51
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!align=left  |Abstract
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Mutants of Escherichia coli which are resistant to 5-fluorodeoxyuridine all have mutations which map at a single locus at 27.5 min on the genetic map of E. coli. Extracts prepared from each mutant were deficient in thymidine kinase activity measured in vitro. Simple selective conditions which allowed detection of one mutant in the presence of 10(7) wild-type bacteria were found. These results show that loss of thymidine kinase activity is the usual mechanism for 5-fluorodeoxyuridine resistance and that all such mutations occur at the locus previously designated tdk.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8376351 PubMed]
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!align=left  |Keywords
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Drug Resistance, Microbial; Escherichia coli; Floxuridine; Genotype; Mutation; Thymidine Kinase
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==Main Points of the Paper ==
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{{LitSignificance}}
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== Materials and Methods Used ==
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{{LitMaterials}}
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==Phenotype Annotations==
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{|  id="I4ffefc6b70ebb"  class=" tableEdit Phenotype_Table_2" 
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!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: C600
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*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
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*Genotype of Reference Strain: ''tdk<sup>+</sup>''
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*Genotype of Experimental Strain : ''tdk'' mutation in C600
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*Reference Condition:
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0000181
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in vitro culture assay data
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Mutation at the ''tdk'' locus caused reduced thymidine kinase activity causing 5-flurodeoxyuridine resistance
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==Notes==
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*All 5-fluorodeoxyuridine resistant strain contained mutations that were mapped to a locus at 27.5 min.
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==References==
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[[Category:Publication]]  [[Category:Papers referenced in the LaRossa chapter]] [[Category:LaRossa citations that need review]]

Latest revision as of 17:35, 7 August 2012

Citation

Summers, WC and Raksin, P (1993) A method for selection of mutations at the tdk locus in Escherichia coli.J. Bacteriol. 175:6049-51

Abstract

Mutants of Escherichia coli which are resistant to 5-fluorodeoxyuridine all have mutations which map at a single locus at 27.5 min on the genetic map of E. coli. Extracts prepared from each mutant were deficient in thymidine kinase activity measured in vitro. Simple selective conditions which allowed detection of one mutant in the presence of 10(7) wild-type bacteria were found. These results show that loss of thymidine kinase activity is the usual mechanism for 5-fluorodeoxyuridine resistance and that all such mutations occur at the locus previously designated tdk.

Links

PubMed

Keywords

Drug Resistance, Microbial; Escherichia coli; Floxuridine; Genotype; Mutation; Thymidine Kinase

edit table

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: C600
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: tdk+
  • Genotype of Experimental Strain : tdk mutation in C600
  • Reference Condition:

0000181

in vitro culture assay data

Mutation at the tdk locus caused reduced thymidine kinase activity causing 5-flurodeoxyuridine resistance


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Notes

  • All 5-fluorodeoxyuridine resistant strain contained mutations that were mapped to a locus at 27.5 min.

References

See Help:References for how to manage references in omp dev.

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