Difference between revisions of "PMID:6337120"

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{|  id="O504a772a871ef"  class=" tableEdit PMID_info_table" 
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!align=left  |Citation
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'''Bogosian, G, Haydock, PV and Somerville, RL'''  (1983) Indolmycin-mediated inhibition and stimulation of transcription at the trp promoter of Escherichia coli.''J. Bacteriol.'' '''153''':1120-3
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!align=left  |Abstract
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Escherichia coli cells harboring a non-attenuated trp-lac operon fusion were used to evaluate the effects of indolmycin on the initiation of transcription at the trp promoter. Indolmycin caused repression in trpR+ strains and in trpR deletion mutants, although higher effector concentrations were required in the latter situation. Plasmid-mediated elevation in tryptophanyl-tRNA synthetase reversed the inhibitory effect of indolmycin. Indolmycin did not facilitate the binding of purified Trp repressor protein to trp operator DNA.
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!align=left  |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6337120 PubMed]
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!align=left  |Keywords
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Bacterial Proteins; DNA, Bacterial; Escherichia coli; Indoles; Mutation; Operon; Repressor Proteins; Transcription, Genetic; Tryptophan; Tryptophan-tRNA Ligase
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==Main Points of the Paper ==
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{{LitSignificance}}
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== Materials and Methods Used ==
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{{LitMaterials}}
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==Phenotype Annotations==
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{{AnnotationTableHelp}}
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{|  id="H504a772a89ccf"  class=" tableEdit Phenotype_Table_2" 
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!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: SP361
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*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
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*Genotype of Reference Strain: ''trpS<sup>+</sup>''
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*Genotype of Experimental Strain : ''trpS'' mutation in SP361
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*Reference Condition:
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0000276
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increased resistance to antibiotics
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0000181
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in vitro assay evidence
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Increase concentration of tryptophanyl-tRNA synthetase caused by the insertion of the second ''trpS'' gene caused increase resistance toward indolmycin.  See Figure 2 for full experimental results.
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a mutation or genetic difference within a strain
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*Taxon: Escherichia coli
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*Strain: K-12
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*Substrain: SP361
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*NCBI Taxon ID: [http://www.ncbi.nlm.nih.gov/taxonomy?term=83333 83333]
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*Genotype of Reference Strain: ''trpR<sup>+</sup>''
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*Genotype of Experimental Strain : ''trpR'' deletion in strain SP361(lambdaGB2)
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*Reference Condition:
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0000276
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increased resistance to antibiotics
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0000181
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in vitro assay evidence
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the deletion of the ''trp'' repressor caused an increase resistance toward Indolmycin. See figure 2 for full experimental results.
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==Notes==
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==References==
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{{RefHelp}}
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<references/>
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[[Category:Publication]] [[Category:Papers referenced in the LaRossa chapter]]

Latest revision as of 17:28, 13 September 2012

Citation

Bogosian, G, Haydock, PV and Somerville, RL (1983) Indolmycin-mediated inhibition and stimulation of transcription at the trp promoter of Escherichia coli.J. Bacteriol. 153:1120-3

Abstract

Escherichia coli cells harboring a non-attenuated trp-lac operon fusion were used to evaluate the effects of indolmycin on the initiation of transcription at the trp promoter. Indolmycin caused repression in trpR+ strains and in trpR deletion mutants, although higher effector concentrations were required in the latter situation. Plasmid-mediated elevation in tryptophanyl-tRNA synthetase reversed the inhibitory effect of indolmycin. Indolmycin did not facilitate the binding of purified Trp repressor protein to trp operator DNA.

Links

PubMed

Keywords

Bacterial Proteins; DNA, Bacterial; Escherichia coli; Indoles; Mutation; Operon; Repressor Proteins; Transcription, Genetic; Tryptophan; Tryptophan-tRNA Ligase

edit table

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SP361
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: trpS+
  • Genotype of Experimental Strain : trpS mutation in SP361
  • Reference Condition:

0000276

increased resistance to antibiotics

0000181

in vitro assay evidence

Increase concentration of tryptophanyl-tRNA synthetase caused by the insertion of the second trpS gene caused increase resistance toward indolmycin. See Figure 2 for full experimental results.

a mutation or genetic difference within a strain

  • Taxon: Escherichia coli
  • Strain: K-12
  • Substrain: SP361
  • NCBI Taxon ID: 83333
  • Genotype of Reference Strain: trpR+
  • Genotype of Experimental Strain : trpR deletion in strain SP361(lambdaGB2)
  • Reference Condition:

0000276

increased resistance to antibiotics

0000181

in vitro assay evidence

the deletion of the trp repressor caused an increase resistance toward Indolmycin. See figure 2 for full experimental results.


edit table

</protect>

Notes

References

See Help:References for how to manage references in omp dev.

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