Difference between revisions of "PMID:1703938"

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'''Van Horn, KG  and Dworkin, BM ''' Direct gram stain and urease test to detect Helicobacter pylori. ''Diagn. Microbiol. Infect. Dis.'' '''13''':449-52
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Antral biopsies were obtained by gastrointestinal endoscopy on 143 adult patients with dyspeptic symptoms of gastritis or peptic ulcer disease. A direct Gram stain and a direct urease test were performed on each biopsy in addition to culture. Forty-three biopsies (30%) were considered positive for Helicobacter pylori based on culture or histologic examination, or both. Thirty-one biopsies (72% sensitivity) were positive for both direct tests, whereas 95 of 100 negative cultures were negative for both tests. Thirty-eight of the 43 positive biopsies were Gram stain positive (sensitivity, 88%; specificity, 100%). The direct urease test alone was positive at 4 hr for 29 biopsies (sensitivity, 67%; specificity, 100%) and at 24 hr for 38 biopsies (sensitivity, 74%; specificity, 95%). Rapid presumptive diagnosis of H. pylori in antral biopsies was obtained when at least one direct test, Gram stain or urease, was positive.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1703938 PubMed]
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Adult; Biopsy; Gastritis/microbiology; Gastroscopy; Gentian Violet; Helicobacter Infections/microbiology; Helicobacter pylori/isolation & purification; Humans; Peptic Ulcer/microbiology; Phenazines; Predictive Value of Tests; Pyloric Antrum; Staining and Labeling; Stomach/microbiology; Urease
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==Main Points of the Paper ==
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== Materials and Methods Used ==
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==Phenotype Annotations==
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==Notes==
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==References==
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[[Category:Publication]]

Latest revision as of 12:00, 17 September 2012

Citation

Van Horn, KG and Dworkin, BM Direct gram stain and urease test to detect Helicobacter pylori. Diagn. Microbiol. Infect. Dis. 13:449-52

Abstract

Antral biopsies were obtained by gastrointestinal endoscopy on 143 adult patients with dyspeptic symptoms of gastritis or peptic ulcer disease. A direct Gram stain and a direct urease test were performed on each biopsy in addition to culture. Forty-three biopsies (30%) were considered positive for Helicobacter pylori based on culture or histologic examination, or both. Thirty-one biopsies (72% sensitivity) were positive for both direct tests, whereas 95 of 100 negative cultures were negative for both tests. Thirty-eight of the 43 positive biopsies were Gram stain positive (sensitivity, 88%; specificity, 100%). The direct urease test alone was positive at 4 hr for 29 biopsies (sensitivity, 67%; specificity, 100%) and at 24 hr for 38 biopsies (sensitivity, 74%; specificity, 95%). Rapid presumptive diagnosis of H. pylori in antral biopsies was obtained when at least one direct test, Gram stain or urease, was positive.

Links

PubMed

Keywords

Adult; Biopsy; Gastritis/microbiology; Gastroscopy; Gentian Violet; Helicobacter Infections/microbiology; Helicobacter pylori/isolation & purification; Humans; Peptic Ulcer/microbiology; Phenazines; Predictive Value of Tests; Pyloric Antrum; Staining and Labeling; Stomach/microbiology; Urease

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Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

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<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status
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</protect>

Notes

References

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