Difference between revisions of "PMID:16095938"
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*Genotype of Reference Strain: ynaI | *Genotype of Reference Strain: ynaI | ||
| − | *Genotype of Experimental Strain : | + | *Genotype of Experimental Strain : ΔynaI ::kan |
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*Reference Condition: BUG agar plates, 30C | *Reference Condition: BUG agar plates, 30C | ||
Revision as of 18:56, 19 April 2012
| Citation |
Ito, M, Baba, T, Mori, H and Mori, H (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays.Metab. Eng. 7:318-27 |
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| Abstract |
Escherichia coli is one of the best elucidated organisms. However, about 40% of E. coli genes have not been assigned to their function yet. We analyzed 1440 single gene knock-out mutants using the GN2-MicroPlate, which permits assay of 95 carbon-source utilizations simultaneously. In the knock-out library there are 1044 of so called y-genes with no apparent function. The raw dataset was analyzed and genes were interrelated by the clustering method of the GeneSpring software. In the resulted dendrogram of genes, a group of genes with known and related function tended to be assembled into a cluster. Our clustering method would be useful for functional assignment of so called y-genes with no apparent function, since the resulted dendrogram could connect y-genes to phenotype and function of well-studied genes. |
| Links |
PubMed Online version:10.1016/j.ymben.2005.06.004 |
| Keywords |
Escherichia coli; Gene Deletion; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Genes, Bacterial; Oligonucleotide Array Sequence Analysis; Phenotype; Software |
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Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
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a mutation or genetic difference within a strain |
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unable to respire N-Acetyl-D-glucosamine,a-D-Glucose-1-phosphate and Acetate |
Biolog |
Mutant shows defective phenotype under N-Acetyl-D-glucosamine,a-D-Glucose-1-phosphate and Acetate chemicals |
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</protect>
Notes
References
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