Difference between revisions of "PMID:8376351"
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| + | {| id="M4ffefc6b6ce5b" class=" tableEdit PMID_info_table" | ||
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| + | !align=left |Citation | ||
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| + | '''Summers, WC and Raksin, P''' (1993) A method for selection of mutations at the tdk locus in Escherichia coli.''J. Bacteriol.'' '''175''':6049-51 | ||
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| + | !align=left |Abstract | ||
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| + | Mutants of Escherichia coli which are resistant to 5-fluorodeoxyuridine all have mutations which map at a single locus at 27.5 min on the genetic map of E. coli. Extracts prepared from each mutant were deficient in thymidine kinase activity measured in vitro. Simple selective conditions which allowed detection of one mutant in the presence of 10(7) wild-type bacteria were found. These results show that loss of thymidine kinase activity is the usual mechanism for 5-fluorodeoxyuridine resistance and that all such mutations occur at the locus previously designated tdk. | ||
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| + | !align=left |Links | ||
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| + | [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8376351 PubMed] | ||
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| + | !align=left |Keywords | ||
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| + | Drug Resistance, Microbial; Escherichia coli; Floxuridine; Genotype; Mutation; Thymidine Kinase | ||
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| + | ==Main Points of the Paper == | ||
| + | {{LitSignificance}} | ||
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| + | == Materials and Methods Used == | ||
| + | {{LitMaterials}} | ||
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| + | ==Phenotype Annotations== | ||
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| + | {| id="I4ffefc6b70ebb" class=" tableEdit Phenotype_Table_2" | ||
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| + | !|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status | ||
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| + | ==Notes== | ||
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| + | ==References== | ||
| + | {{RefHelp}} | ||
| + | <references/> | ||
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| + | [[Category:Publication]] | ||
Revision as of 11:33, 12 July 2012
| Citation |
Summers, WC and Raksin, P (1993) A method for selection of mutations at the tdk locus in Escherichia coli.J. Bacteriol. 175:6049-51 |
|---|---|
| Abstract |
Mutants of Escherichia coli which are resistant to 5-fluorodeoxyuridine all have mutations which map at a single locus at 27.5 min on the genetic map of E. coli. Extracts prepared from each mutant were deficient in thymidine kinase activity measured in vitro. Simple selective conditions which allowed detection of one mutant in the presence of 10(7) wild-type bacteria were found. These results show that loss of thymidine kinase activity is the usual mechanism for 5-fluorodeoxyuridine resistance and that all such mutations occur at the locus previously designated tdk. |
| Links | |
| Keywords |
Drug Resistance, Microbial; Escherichia coli; Floxuridine; Genotype; Mutation; Thymidine Kinase |
| edit table |
Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
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<protect>
| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
| edit table |
</protect>
Notes
References
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