Difference between revisions of "PMID:8722757"

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'''Sandler, SJ, Samra, HS and Clark, AJ'''  (1996) Differential suppression of priA2::kan phenotypes in Escherichia coli K-12 by mutations in priA, lexA, and dnaC. ''Genetics'' '''143''':5-13
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!align=left align='left' bgcolor='#CCCCFF' |Abstract
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First identified as an essential component of the phi X174 in vitro DNA replication system, PriA has ATPase, helicase, translocase, and primosome-assembly activities. priA1::kan strains of Escherichia coli are sensitive to UV irradiation, deficient in homologous recombination following transduction, and filamentous. priA2::kan strains have eightfold higher levels of uninduced SOS expression than wild type. We show that (1) priA1::kan strains have eightfold higher levels of uninduced SOS expression, (2) priA2::kan strains are UVS and Rec-, (3) lexA3 suppresses the high basal levels of SOS expression of a priA2::kan strain, and (4) plasmid-encoded priA300 (K230R), a mutant allele retaining only the primosome-assembly activity of priA+, restores both UVR and Rec+ phenotypes to a priA2::kan strain. Finally, we have isolated 17 independent UVR Rec+ revertants of priA2::kan strains that carry extragenic suppressors. All 17 map in the C-terminal half of the dnaC gene. DnaC loads the DnaB helicase onto DNA as a prelude for primosome assembly and DNA replication. We conclude that priA's primosome-assembly activity is essential for DNA repair and recombination and that the dnaC suppressor mutations allow these processes to occur in the absence of priA.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8722757 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1207281 PMC1207281]
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!align=left align='left' bgcolor='#CCCCFF' |Keywords
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Bacterial Proteins/biosynthesis; Bacterial Proteins/genetics; Bacteriophage phi X 174/genetics; Chromosomes, Bacterial; DNA Helicases/genetics; DNA Replication; DNA-Binding Proteins/biosynthesis; DNA-Binding Proteins/genetics; Dose-Response Relationship, Radiation; Escherichia coli/genetics; Escherichia coli/radiation effects; Escherichia coli Proteins; Genes, Bacterial/radiation effects; Genetic Markers; Mutagenesis; Phenotype; Recombination, Genetic; Replication Protein A; Repressor Proteins/genetics; Serine Endopeptidases/biosynthesis; Serine Endopeptidases/genetics; Suppression, Genetic; Transduction, Genetic; Ultraviolet Rays; beta-Galactosidase/biosynthesis
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==Main Points of the Paper ==
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== Materials and Methods Used ==
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==Phenotype Annotations==
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==Notes==
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==References==
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[[Category:Publication]]

Latest revision as of 15:26, 21 July 2015

Citation

Sandler, SJ, Samra, HS and Clark, AJ (1996) Differential suppression of priA2::kan phenotypes in Escherichia coli K-12 by mutations in priA, lexA, and dnaC. Genetics 143:5-13

Abstract

First identified as an essential component of the phi X174 in vitro DNA replication system, PriA has ATPase, helicase, translocase, and primosome-assembly activities. priA1::kan strains of Escherichia coli are sensitive to UV irradiation, deficient in homologous recombination following transduction, and filamentous. priA2::kan strains have eightfold higher levels of uninduced SOS expression than wild type. We show that (1) priA1::kan strains have eightfold higher levels of uninduced SOS expression, (2) priA2::kan strains are UVS and Rec-, (3) lexA3 suppresses the high basal levels of SOS expression of a priA2::kan strain, and (4) plasmid-encoded priA300 (K230R), a mutant allele retaining only the primosome-assembly activity of priA+, restores both UVR and Rec+ phenotypes to a priA2::kan strain. Finally, we have isolated 17 independent UVR Rec+ revertants of priA2::kan strains that carry extragenic suppressors. All 17 map in the C-terminal half of the dnaC gene. DnaC loads the DnaB helicase onto DNA as a prelude for primosome assembly and DNA replication. We conclude that priA's primosome-assembly activity is essential for DNA repair and recombination and that the dnaC suppressor mutations allow these processes to occur in the absence of priA.

Links

PubMed PMC1207281

Keywords

Bacterial Proteins/biosynthesis; Bacterial Proteins/genetics; Bacteriophage phi X 174/genetics; Chromosomes, Bacterial; DNA Helicases/genetics; DNA Replication; DNA-Binding Proteins/biosynthesis; DNA-Binding Proteins/genetics; Dose-Response Relationship, Radiation; Escherichia coli/genetics; Escherichia coli/radiation effects; Escherichia coli Proteins; Genes, Bacterial/radiation effects; Genetic Markers; Mutagenesis; Phenotype; Recombination, Genetic; Replication Protein A; Repressor Proteins/genetics; Serine Endopeptidases/biosynthesis; Serine Endopeptidases/genetics; Suppression, Genetic; Transduction, Genetic; Ultraviolet Rays; beta-Galactosidase/biosynthesis

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Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status
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Notes

References

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