Difference between revisions of "PMID:7047497"
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+ | {| id="L4f6399050ef53" class=" tableEdit PMID_info_table" | ||
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+ | !align=left |Citation | ||
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+ | '''Stewart, V and MacGregor, CH''' (1982) Nitrate reductase in Escherichia coli K-12: involvement of chlC, chlE, and chlG loci.''J. Bacteriol.'' '''151''':788-99 | ||
+ | |- | ||
+ | !align=left |Abstract | ||
+ | || | ||
+ | We examined the properties of mutants of E. coli which are defective with respect to nitrate reductase activity. chlE::Mu cts and chlG::Mu cts mutants were all chlorate resistant, and the strains that we examined all synthesized nitrate reductase apoenzyme. We concluded that the chlE and chlG loci, like the chlA, chlB, and chlD loci, are involved in the synthesis of insertion of molybdenum cofactor. We identified at least four distinct phenotypic classes of chlC::Tn10 mutants, all of which were fully or partially sensitive to chlorate. Two of these classes may represent lesions in the structural genes for nitrate reductase subunits A and C. Two other classes may be altered in the regulation of the expression of nitrate reductase or other anaerobic enzymes. We propose the mnemonic nar for naming individual genes within the chlC locus. | ||
+ | |- | ||
+ | !align=left |Links | ||
+ | || | ||
+ | [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7047497 PubMed] | ||
+ | |||
+ | |- | ||
+ | !align=left |Keywords | ||
+ | || | ||
+ | Apoenzymes; Chlorates; Coenzymes; Escherichia coli; Genes; Genes, Bacterial; Metalloproteins; Molybdenum; Mutation; Nitrate Reductases; Pteridines | ||
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+ | <!--box uid=d41d8cd98f00b204e9800998ecf8427e.2876.L4f6399050ef53--> | ||
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+ | ==Main Points of the Paper == | ||
+ | {{LitSignificance}} | ||
+ | |||
+ | == Materials and Methods Used == | ||
+ | {{LitMaterials}} | ||
+ | |||
+ | ==Phenotype Annotations== | ||
+ | {{AnnotationTableHelp}} | ||
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+ | {| id="W4f6399052f2dc" class=" tableEdit Phenotype_Table_2" | ||
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+ | !|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status | ||
+ | |||
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+ | |} | ||
+ | <!--box uid=d41d8cd98f00b204e9800998ecf8427e.2876.W4f6399052f2dc--></protect> | ||
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+ | ==Notes== | ||
+ | |||
+ | ==References== | ||
+ | {{RefHelp}} | ||
+ | <references/> | ||
+ | |||
+ | |||
+ | [[Category:Publication]] |
Revision as of 14:48, 16 March 2012
Citation |
Stewart, V and MacGregor, CH (1982) Nitrate reductase in Escherichia coli K-12: involvement of chlC, chlE, and chlG loci.J. Bacteriol. 151:788-99 |
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Abstract |
We examined the properties of mutants of E. coli which are defective with respect to nitrate reductase activity. chlE::Mu cts and chlG::Mu cts mutants were all chlorate resistant, and the strains that we examined all synthesized nitrate reductase apoenzyme. We concluded that the chlE and chlG loci, like the chlA, chlB, and chlD loci, are involved in the synthesis of insertion of molybdenum cofactor. We identified at least four distinct phenotypic classes of chlC::Tn10 mutants, all of which were fully or partially sensitive to chlorate. Two of these classes may represent lesions in the structural genes for nitrate reductase subunits A and C. Two other classes may be altered in the regulation of the expression of nitrate reductase or other anaerobic enzymes. We propose the mnemonic nar for naming individual genes within the chlC locus. |
Links | |
Keywords |
Apoenzymes; Chlorates; Coenzymes; Escherichia coli; Genes; Genes, Bacterial; Metalloproteins; Molybdenum; Mutation; Nitrate Reductases; Pteridines |
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Main Points of the Paper
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Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
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Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
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Notes
References
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