Difference between revisions of "PMID:21183718"

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'''Washburn, RS and Gottesman, ME'''  (2011) Transcription termination maintains chromosome integrity.''Proc. Natl. Acad. Sci. U.S.A.'' '''108''':792-7
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!align=left  |Abstract
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DNA replication fork movement is impeded by collisions with transcription elongation complexes (TEC). We propose that a critical function of transcription termination factors is to prevent TEC from blocking DNA replication and inducing replication fork arrest, one consequence of which is DNA double-strand breaks. We show that inhibition of Rho-dependent transcription termination by bicyclomycin in Escherichia coli induced double-strand breaks. Cells deleted for Rho-cofactors nusA and nusG were hypersensitive to bicyclomycin, and had extensive chromosome fragmentation even in the absence of the drug. An RNA polymerase mutation that destabilizes TEC (rpoB*35) increased bicyclomycin resistance >40-fold. Double-strand break formation depended on DNA replication, and can be explained by replication fork collapse. Deleting recombination genes required for replication fork repair (recB and ruvC) increased sensitivity to bicyclomycin, as did loss of the replication fork reloading helicases rep and priA. We propose that Rho responds to a translocating replisome by releasing obstructing TEC.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=21183718 PubMed]
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Online version:[http://dx.doi.org/10.1073/pnas.1009564108 10.1073/pnas.1009564108]
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Bicyclo Compounds, Heterocyclic; Chromosomes; DNA Mutational Analysis; DNA Replication; DNA-Directed DNA Polymerase; DNA-Directed RNA Polymerases; Escherichia coli; Escherichia coli Proteins; Models, Genetic; Mutation; Peptide Elongation Factors; Transcription Factors; Transcription, Genetic; Ultraviolet Rays
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==Main Points of the Paper ==
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== Materials and Methods Used ==
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{{LitMaterials}}
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==Phenotype Annotations==
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{|  id="G4f70c487e1386"  class=" tableEdit Phenotype_Table_2" 
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!|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status
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==Notes==
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==References==
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[[Category:Publication]]

Latest revision as of 14:33, 26 March 2012

Citation

Washburn, RS and Gottesman, ME (2011) Transcription termination maintains chromosome integrity.Proc. Natl. Acad. Sci. U.S.A. 108:792-7

Abstract

DNA replication fork movement is impeded by collisions with transcription elongation complexes (TEC). We propose that a critical function of transcription termination factors is to prevent TEC from blocking DNA replication and inducing replication fork arrest, one consequence of which is DNA double-strand breaks. We show that inhibition of Rho-dependent transcription termination by bicyclomycin in Escherichia coli induced double-strand breaks. Cells deleted for Rho-cofactors nusA and nusG were hypersensitive to bicyclomycin, and had extensive chromosome fragmentation even in the absence of the drug. An RNA polymerase mutation that destabilizes TEC (rpoB*35) increased bicyclomycin resistance >40-fold. Double-strand break formation depended on DNA replication, and can be explained by replication fork collapse. Deleting recombination genes required for replication fork repair (recB and ruvC) increased sensitivity to bicyclomycin, as did loss of the replication fork reloading helicases rep and priA. We propose that Rho responds to a translocating replisome by releasing obstructing TEC.

Links

PubMed Online version:10.1073/pnas.1009564108

Keywords

Bicyclo Compounds, Heterocyclic; Chromosomes; DNA Mutational Analysis; DNA Replication; DNA-Directed DNA Polymerase; DNA-Directed RNA Polymerases; Escherichia coli; Escherichia coli Proteins; Models, Genetic; Mutation; Peptide Elongation Factors; Transcription Factors; Transcription, Genetic; Ultraviolet Rays

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Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status
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Notes

References

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