Difference between revisions of "PMID:3918006"

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{|  id="Q4fb3f2b242ff5"  class=" tableEdit PMID_info_table" 
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!align=left  |Citation
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'''Sullivan, MA and Bock, RM'''  (1985) Isolation and characterization of antisuppressor mutations in Escherichia coli.''J. Bacteriol.'' '''161''':377-84
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!align=left  |Abstract
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Nonsense mutations in lacI have been shown to be useful as indicators of the efficiency of nonsense suppression. From strains containing supE and a lacI nonsense mutation, selection for LacI- mutants has resulted in the isolation of four antisuppressor mutations. Tn10 insertions linked to these mutations were isolated and used to group the four mutations into three loci. The asuA1 and asuA2 mutations are linked to trp, reduce suppression by supE approximately twofold, and affect a variety of suppressors. The asuB3 mutation was mapped by P1 cotransduction to rpsL but does not confer resistance to streptomycin. The asuC4 mutation reduced suppression by supE by 95% and was shown biochemically to result in the loss of two pseudouridine modifications from the 3' side of the anticodon stem and loop of tRNA2Gln. This mutation is linked to purF, suggesting that it is a new allele of hisT.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3918006 PubMed]
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!align=left  |Keywords
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Base Sequence; Codon; Escherichia coli; Genes, Bacterial; Lac Operon; Mutation; Pseudouridine; RNA, Transfer; RNA, Transfer, Amino Acyl; Suppression, Genetic; Transduction, Genetic; beta-Galactosidase
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==Main Points of the Paper ==
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{{LitSignificance}}
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== Materials and Methods Used ==
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{{LitMaterials}}
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==Phenotype Annotations==
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==Notes==
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==References==
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[[Category:Publication]]

Revision as of 13:32, 16 May 2012

Citation

Sullivan, MA and Bock, RM (1985) Isolation and characterization of antisuppressor mutations in Escherichia coli.J. Bacteriol. 161:377-84

Abstract

Nonsense mutations in lacI have been shown to be useful as indicators of the efficiency of nonsense suppression. From strains containing supE and a lacI nonsense mutation, selection for LacI- mutants has resulted in the isolation of four antisuppressor mutations. Tn10 insertions linked to these mutations were isolated and used to group the four mutations into three loci. The asuA1 and asuA2 mutations are linked to trp, reduce suppression by supE approximately twofold, and affect a variety of suppressors. The asuB3 mutation was mapped by P1 cotransduction to rpsL but does not confer resistance to streptomycin. The asuC4 mutation reduced suppression by supE by 95% and was shown biochemically to result in the loss of two pseudouridine modifications from the 3' side of the anticodon stem and loop of tRNA2Gln. This mutation is linked to purF, suggesting that it is a new allele of hisT.

Links

PubMed

Keywords

Base Sequence; Codon; Escherichia coli; Genes, Bacterial; Lac Operon; Mutation; Pseudouridine; RNA, Transfer; RNA, Transfer, Amino Acyl; Suppression, Genetic; Transduction, Genetic; beta-Galactosidase

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Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

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<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status
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</protect>

Notes

References

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