PMID:6802799

Citation	Newman, EB, Malik, N and Walker, C (1982) L-serine degradation in Escherichia coli K-12: directly isolated ssd mutants and their intragenic revertants.J. Bacteriol. 150:710-5
Abstract	Two methods for the direct isolation of spontaneous ssd mutants of Escherichia coli K-12 strains are described; (i) by growth with L-serine as the carbon source, and (ii) by low-level kanamycin resistance. A newly isolated mutant had the same phenotype as the mutant described previously, including inefficient use of the glucose, inability to growth with succinate, altered transport characteristics, and altered resistance to certain growth effectors. Succinate-utilizing derivatives which appear to be intragenic are characterized in detail. The relation between the mutants isolated here and mutants which are thought to have impairment in a system of coupling respiratory energy to active transport (ecfB mutants) is discussed.
Links	PubMed
Keywords	Biological Transport, Active; Drug Resistance, Microbial; Escherichia coli; Genes, Bacterial; Glucose; Kanamycin; Mutation; Serine; Succinates; Succinic Acid
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Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: CU1008 NCBI Taxon ID: 83333	Genotype of Reference Strain: ecfB Genotype of Experimental Strain : ecfB in strains KEC1 - KEC20	Reference Condition:	OMP:0000266	utilizes carbon-containing compound			Carbon utilization of L-Serine. See table 1
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: W4977 NCBI Taxon ID: 83333	Genotype of Reference Strain: ecfB Genotype of Experimental Strain : ecfB in strains KEC21 - KEC30	Reference Condition:	OMP:0000266	utilizes carbon-containing compound			Carbon utilization of L-Serine. See table 1
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: CU1008 NCBI Taxon ID: 83333	Genotype of Reference Strain: ssd Genotype of Experimental Strain : ssd' in mutant strain KEC3	Reference Condition:					Resistance to Kanamycin
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: CU1008 NCBI Taxon ID: 83333	Genotype of Reference Strain: ssd Genotype of Experimental Strain : ssd' in mutant strain KEC3	Reference Condition:					Resistance to Neomycin
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Notes

• L-serine phenotype:
  • ECOID: This is a solid medium assay in minimal medium.

References

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