Difference between revisions of "PMID:1551857"
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+ | {| id="R50576fd02ea2e" class=" tableEdit PMID_info_table" | ||
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+ | !align=left |Citation | ||
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+ | '''Beall, B and Lutkenhaus, J ''' (1992) Impaired cell division and sporulation of a Bacillus subtilis strain with the ftsA gene deleted. ''J. Bacteriol.'' '''174''':2398-403 | ||
+ | |- | ||
+ | !align=left |Abstract | ||
+ | || | ||
+ | The ftsZ and ftsA genes of Bacillus subtilis are organized in a simple operon expressed from promoter sequences immediately upstream of ftsA. The promoter-distal ftsZ gene is an essential septation gene. In this report, it is shown that the promoter-proximal ftsA gene can be deleted in a previously constructed strain in which the essential gene, ftsZ, is under the control of the inducible spac promoter. Absence of the ftsA gene product resulted in a very filamentous morphology indicating an important role for ftsA in cell division. Also, growth was severely impaired, and viability and sporulation were reduced. The defective sporulation phenotype correlated with a deficiency in the processing of pro-sigma E to its active form. | ||
+ | |- | ||
+ | !align=left |Links | ||
+ | || | ||
+ | [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1551857 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC205866 PMC205866] | ||
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+ | |- | ||
+ | !align=left |Keywords | ||
+ | || | ||
+ | Bacillus subtilis/cytology; Bacillus subtilis/genetics; Cell Division; Chromosome Deletion; DNA Mutational Analysis; DNA, Bacterial/genetics; Genes, Bacterial; Restriction Mapping; Spores, Bacterial | ||
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+ | <!--box uid=d41d8cd98f00b204e9800998ecf8427e.3495.R50576fd02ea2e--> | ||
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+ | ==Main Points of the Paper == | ||
+ | {{LitSignificance}} | ||
+ | |||
+ | == Materials and Methods Used == | ||
+ | {{LitMaterials}} | ||
+ | |||
+ | ==Phenotype Annotations== | ||
+ | {{AnnotationTableHelp}} | ||
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+ | !|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status | ||
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+ | <!--box uid=d41d8cd98f00b204e9800998ecf8427e.3495.A50576fd036979--></protect> | ||
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+ | ==Notes== | ||
+ | |||
+ | ==References== | ||
+ | {{RefHelp}} | ||
+ | <references/> | ||
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+ | |||
+ | [[Category:Publication]] |
Latest revision as of 13:45, 17 September 2012
Citation |
Beall, B and Lutkenhaus, J (1992) Impaired cell division and sporulation of a Bacillus subtilis strain with the ftsA gene deleted. J. Bacteriol. 174:2398-403 |
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Abstract |
The ftsZ and ftsA genes of Bacillus subtilis are organized in a simple operon expressed from promoter sequences immediately upstream of ftsA. The promoter-distal ftsZ gene is an essential septation gene. In this report, it is shown that the promoter-proximal ftsA gene can be deleted in a previously constructed strain in which the essential gene, ftsZ, is under the control of the inducible spac promoter. Absence of the ftsA gene product resulted in a very filamentous morphology indicating an important role for ftsA in cell division. Also, growth was severely impaired, and viability and sporulation were reduced. The defective sporulation phenotype correlated with a deficiency in the processing of pro-sigma E to its active form. |
Links | |
Keywords |
Bacillus subtilis/cytology; Bacillus subtilis/genetics; Cell Division; Chromosome Deletion; DNA Mutational Analysis; DNA, Bacterial/genetics; Genes, Bacterial; Restriction Mapping; Spores, Bacterial |
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Main Points of the Paper
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Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
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<protect>
Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
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Notes
References
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