PMID:9973363
Citation |
Dermić, D and Trgovcević, Z (1999) Specific effects of a recB mutation on the HfrH strain of Escherichia coli.J. Bacteriol. 181:1334-7 |
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Abstract |
The recB268::Tn10 mutation was introduced into the HfrH strain of Escherichia coli. Compared with recB F- and recB F+ cells, the viability of this mutant strain was much lower. Compared with wild-type HfrH, the recB derivative donated much shorter fragments of its chromosome to the recipient. It is suggested that the recB gene product (i.e., RecBCD enzyme) participates in Hfr transfer. |
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Keywords |
Conjugation, Genetic; Escherichia coli; Escherichia coli Proteins; Exodeoxyribonuclease V; Exodeoxyribonucleases; F Factor; Mutation |
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Main Points of the Paper
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Materials and Methods Used
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Phenotype Annotations
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Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
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a mutation or genetic difference within a strain |
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After the insertion of the Tn10 mutation the IRB101 cells grew extremely slowly. The generation time being four to five times longer than the parental strain's. |
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