id: ECO:0000074
name: split-ubiquitin assay evidence
def: "Used when an annotation is based on the interaction of two proteins in a split-ubiquitin assay. The bait protein is fused to the C-terminal ubiquitin (Cub) domain followed by a reporter protein, and the prey protein is fused to a mutated N terminal ubiquiting (NubG) domain. If bait and prey interact, their interaction brings the NubG and Cub domains close enough together to reconstitute split-ubiquitin, resulting in the release of the reporter protein by the action of the UBPs." [TAIR:TED]
synonym: "IPI: split-ubiquitin assay" RELATED []
xref: [ MI:0112 "ubiquitin reconstruction"]
is_a: ECO:0000021 ! physical interaction evidence
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