PMID:342516

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Citation

Long, WS, Slayman, CL and Low, KB (1978) Production of giant cells of Escherichia coli.J. Bacteriol. 133:995-1007

Abstract

Giant cells, with volumes up to 500-fold those of normal cells, have been produced by both genetic and pharmacological means in Escherichia coli K-12. In the genetic approach, an envB or mon mutation (conferring rounded or irregular morphology) was combined with a lon mutation (block of septation after irradiation). UV irradiation and subsequent incubation for 2 to 5 h in a rich medium supplemented with 1% sodium chloride led t; production of polymorphic giant cells. In the pharmacological approach, incubation of several different strains of E. coli K-12 with the drug 6-amidinopenicillanic acid (FL1060) in the same rich medium gave rise to a homogeneous population of smoothly rounded giant cells.

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Keywords

Cell Division; Escherichia coli; Microscopy, Phase-Contrast; Mutation; Phenotype; Transduction, Genetic; Ultraviolet Rays

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

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