PMID:3543211
| Citation |
Laviña, M, Pugsley, AP and Moreno, F (1986) Identification, mapping, cloning and characterization of a gene (sbmA) required for microcin B17 action on Escherichia coli K12.J. Gen. Microbiol. 132:1685-93 |
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| Abstract |
We have identified mutations in three different chromosomal genes of Escherichia coli K12 which reduce sensitivity to microcin B17. Mutations in ompF and ompR genes affected production of an outer membrane porin protein, OmpF, and resulted in reduced sensitivity to a number of other agents (colicins, bacteriophages) besides microcin B17. The third class of mutants were specifically and highly resistant to microcin B17. The mutations in these strains were mapped to a gene (sbmA), located at 8.7 min on the E. coli K12 chromosome, which is closely linked to phoA. The wild-type sbmA allele was cloned into multiple copy number plasmids, and its location within the cloned DNA fragment was further defined by mutagenesis with MiniMudII1681. These insertion mutations resulted in in-frame fusions between the sbmA and lacZ genes, thereby allowing us to determine the direction of sbmA gene transcription. Plasmids carrying these gene fusions produced low levels of beta-galactosidase, indicating that the sbmA gene is poorly expressed. We have been unable to identify the sbmA gene product, but indirect evidence indicates that it might be an envelope protein involved in microcin uptake. |
| Links | |
| Keywords |
Anti-Bacterial Agents; Bacteriocins; Chromosome Mapping; Cloning, Molecular; Escherichia coli; Genes, Bacterial; Mutation; Phenotype; Transduction, Genetic |
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Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
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a mutation or genetic difference within a strain |
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|
0000276 |
increased resistance to antibiotics |
0000033 |
traceable author statement |
Insertion of transposon into sbmA gene increased the occurrence Microcin B17 resistance. Author statement on page 1686 |
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a mutation or genetic difference within a strain |
|
|
|
0000276 |
increased resistance to antibiotics |
0000033 |
traceable author statement |
Insertion of transposon into sbmA gene increased the occurrence Microcin B17 resistance and Kanamycin resistance. Author statement on page 1686 |
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|
a mutation or genetic difference within a strain |
|
|
|
0000276 |
increased resistance to antibiotics |
0000181 |
in vitro assay data |
The introduction ompR mutation causes a partial increase in resistance toward microcin B17. See Table 4 for full range of Microcin B17 sensitivity experiment results. |
|
|
a mutation or genetic difference within a strain |
|
|
|
0000276 |
increased resistance to antibiotics |
0000181 |
in vitro assay data |
The introduction ompF mutation causes a partial increase in resistance toward microcin B17. See Table 4 for full range of Microcin B17 sensitivity experiment results. |
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|
a mutation or genetic difference within a strain |
|
|
|
0000276 |
increased resistance to antibiotics |
0000181 |
in vitro assay data |
The introduction ompR and recA mutation causes a partial increase in resistance toward microcin B17. See Table 4 for full range of Microcin B17 sensitivity experiment results. |
|
|
a mutation or genetic difference within a strain |
|
|
|
0000276 |
increased resistance to antibiotics |
0000181 |
in vitro assay data |
The introduction ompF and recA mutation causes a partial increase in resistance toward microcin B17. See Table 4 for full range of Microcin B17 sensitivity experiment results. |
|
|
a mutation or genetic difference within a strain |
|
|
|
0000275 |
decreased resistance to antibiotics |
0000181 |
in vitro assay data |
Insertion of recA mutant increased sensitivity toward microcin B17. See table 4 for full experimental results. |
|
|
a mutation or genetic difference within a strain |
|
|
|
0000276 |
Increased resistance to antibiotics |
0000181 |
in vitro assay data |
Insertion of ompC mutation causes a slight decrease in microcin B17 sensitivity. See table 4 for full experimental results. |
|
|
a mutation or genetic difference within a strain |
|
|
|
0000276 |
increased resistance to antibiotics |
0000181 |
in vitro assay data |
Insertion of sbmA mutation caused complete resistance to microcin B17. See table 4. |
|
|
a mutation or genetic difference within a strain |
|
|
|
0000276 |
increased resistance to antibiotics |
0000181 |
in vitro assay data |
Insertion of sbmA mutation caused complete resistance to microcin B17 even with the recA mutation. See table 4. |
|
| edit table |
</protect>
Notes
References
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