id: ECO:0000223
name: 454 pyrosequencing evidence
def: "A high throughput nucleotide sequencing result derived by 454 pyrosequencing technology." [ECO:MCC]
synonym: "pyrosequencing" BROAD [OBI:0000730]
is_a: ECO:0000221 ! high throughput nucleotide sequencing assay evidence
comment: 454 pyrosequencing technology amplifies individual DNA fragments inside water droplets within an oil solution (emulsion PCR). Within each droplet, a single DNA template is attached to a single primer-coated bead. Each bead is subsequently captured in a picolitre well within the sequencing machine. A luciferase-based readout system is used to identify individual nucleotides added to the nascent DNA.
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