PMID:18627634

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Citation

Flaberg, E , Sabelström, P , Strandh, C and Szekely, L (2008) Extended Field Laser Confocal Microscopy (EFLCM): combining automated Gigapixel image capture with in silico virtual microscopy. BMC Med Imaging 8:13

Abstract

Confocal laser scanning microscopy has revolutionized cell biology. However, the technique has major limitations in speed and sensitivity due to the fact that a single laser beam scans the sample, allowing only a few microseconds signal collection for each pixel. This limitation has been overcome by the introduction of parallel beam illumination techniques in combination with cold CCD camera based image capture.

Links

PubMed PMC2515298 Online version:10.1186/1471-2342-8-13

Keywords

Animals; Artificial Intelligence; Equipment Design; Equipment Failure Analysis; Image Enhancement/instrumentation; Image Interpretation, Computer-Assisted/instrumentation; Microscopy, Confocal/instrumentation; Pattern Recognition, Automated/methods; Phantoms, Imaging; Rats; Reproducibility of Results; Sensitivity and Specificity; Signal Processing, Computer-Assisted/instrumentation; User-Computer Interface

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